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@proceedings{571289,
author = {David, Marek and Gabriel, Miroslav and Kopecká, Marie},
booktitle = {XIII. Cytoskeletální klub, Hotel SKI, Nové Město na Moravě 27.-29.4.2005},
keywords = {basidiomycetous yeast; Cryptococcus laurentii; cytoskeleton; F-actin; microtubules},
language = {eng},
title = {Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii},
url = {http://www.med.muni.cz/biolspol/cytoklub/index.html},
year = {2005}
}
TY - CONF
ID - 571289
AU - David, Marek - Gabriel, Miroslav - Kopecká, Marie
PY - 2005
TI - Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii
KW - basidiomycetous yeast
KW - Cryptococcus laurentii
KW - cytoskeleton
KW - F-actin
KW - microtubules
UR - http://www.med.muni.cz/biolspol/cytoklub/index.html
N2 - The cytoskeleton was investigated in Cryptococcus laurentii. The actin cytoskeleton stained by rhodamine-phalloidin in encapsulated strain CCY 17-3-5 failed, but the actin patches were detected by application of monoclonal anti-actin antibody and secondary antibody SwAM-FITC. In acapsular strain C. laurentii 17-3-6, F-actin was stainable by rhodamine-phalloidin. The cortical actin patches were distributed under cell surface and actin cables extended to the bud. At cytokinesis, the actin cables were replaced with contractile ring. Microtubules were seen in both yeast strains. Cytoplasmatic microtubules during interphase arranged into a random network under the plasma membrane. Before mitosis, nucleus migrated from the mother cell to the bud, cytoplasmatic microtubules were disassembled, the spindle formed that allowed the nuclear division. Before the cytokinesis the cytoplasmatic microtubules reappeared. The dynamics of cytoskeleton in C. laurentii is similar to Cryptococcus neoformans (2).
ER -
DAVID, Marek, Miroslav GABRIEL a Marie KOPECKÁ. Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii. In \textit{XIII. Cytoskeletální klub, Hotel SKI, Nové Město na Moravě 27.-29.4.2005}. 2005.
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