Detailed Information on Publication Record
2003
Comparative genomics of pathogenic Treponema pallidum species
MATĚJKOVÁ, Petra, David ŠMAJS, SJ NORRIS and GM WEINSTOCKBasic information
Original name
Comparative genomics of pathogenic Treponema pallidum species
Name in Czech
Komparativní genomika patogenních druhů Treponema pallidum
Name (in English)
Comparative genomics of pathogenic Treponema pallidum species
Authors
MATĚJKOVÁ, Petra (203 Czech Republic), David ŠMAJS (203 Czech Republic, guarantor), SJ NORRIS (840 United States of America) and GM WEINSTOCK (840 United States of America)
Edition
2003. vyd. Brno, Sborník příspěvků VII. Pracovní setkání biochemiků a molekulárních biologů, p. 3 - 4, 1 pp. 2003
Publisher
Masarykova univerzita
Other information
Language
Czech
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14110/03:00012306
Organization unit
Faculty of Medicine
ISBN
80-210-3053-4
Keywords in English
comparative genomics; Treponema pallidum
Změněno: 31/5/2005 09:29, prof. MUDr. David Šmajs, Ph.D.
V originále
Spirochaetal genus Treponema includes several pathogenic spirochetes (e.g. Treponema pallidum subsp. pallidum is the causative agent of syphilis, T. pallidum subsp. pertenue causes yaws). Recent serological tests are negative in early stages of treponemal infection and cannot distinguish between syphilis and yaws. The complete genome sequence, construction of a microarray chip with all 1039 predicted ORF PCR products, together with the findings that there is a high degree of sequence homology among pathogenic treponemes, enables comparative genomic analyses based on DNA-microarray techniques. Identification of chromosomal sequences specific for these pathogens can be used for selective PCR diagnostics of treponemal diseases. DNA of Treponema pallidum subsp. pallidum Nichols strain was compared to DNA isolated from three different strains of T. pallidum subsp. pertenue (strain Gauthier, Samoan D, CDC-2). As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
In English
Spirochaetal genus Treponema includes several pathogenic spirochetes (e.g. Treponema pallidum subsp. pallidum is the causative agent of syphilis, T. pallidum subsp. pertenue causes yaws). Recent serological tests are negative in early stages of treponemal infection and cannot distinguish between syphilis and yaws. The complete genome sequence, construction of a microarray chip with all 1039 predicted ORF PCR products, together with the findings that there is a high degree of sequence homology among pathogenic treponemes, enables comparative genomic analyses based on DNA-microarray techniques. Identification of chromosomal sequences specific for these pathogens can be used for selective PCR diagnostics of treponemal diseases. DNA of Treponema pallidum subsp. pallidum Nichols strain was compared to DNA isolated from three different strains of T. pallidum subsp. pertenue (strain Gauthier, Samoan D, CDC-2). As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
Links
| NI7351, research and development project |
|