MATĚJKOVÁ, Petra and David ŠMAJS. Komparativní genomika patogenních poddruhů Treponema pallidum (Comparative genomics of pathogenic Treponema pallidum subspecies). In Abstrakta 47. Studentské vědecké konference. 2003. vyd. Brno: Lékařská fakulta MU, 2003. p. 15 - 16, 1 pp.
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Basic information
Original name Komparativní genomika patogenních poddruhů Treponema pallidum
Name in Czech Komparativní genomika patogenních poddruhů Treponema pallidum
Name (in English) Comparative genomics of pathogenic Treponema pallidum subspecies
Authors MATĚJKOVÁ, Petra (203 Czech Republic) and David ŠMAJS (203 Czech Republic, guarantor).
Edition 2003. vyd. Brno, Abstrakta 47. Studentské vědecké konference, p. 15 - 16, 1 pp. 2003.
Publisher Lékařská fakulta MU
Other information
Original language Czech
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/03:00012308
Organization unit Faculty of Medicine
Keywords in English comparative genomics; Treponema pallidum
Tags comparative genomics, Treponema pallidum
Changed by Changed by: prof. MUDr. David Šmajs, Ph.D., učo 1116. Changed: 31/5/2005 10:23.
Abstract
As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
Abstract (in English)
As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
Links
NI7351, research and development projectName: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice
Investor: Ministry of Health of the CR, Infectious Diseases, Microbiology, Epidemiology and Immunology
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