MATĚJKOVÁ, Petra, David ŠMAJS, SJ NORRIS and GM WEINSTOCK. Comparative genomics of pathogenic Treponema pallidum subspecies. CHEMICKÉ LISTY, Praha: Česká společnost chemická, 2003, vol. 2003, No 97, p. 297-298. ISSN 0009-2770.
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Basic information
Original name Comparative genomics of pathogenic Treponema pallidum subspecies
Name in Czech Komparativní genomika patogenních poddruhů Treponema pallidum
Authors MATĚJKOVÁ, Petra (203 Czech Republic), David ŠMAJS (203 Czech Republic, guarantor), SJ NORRIS (840 United States of America) and GM WEINSTOCK (840 United States of America).
Edition CHEMICKÉ LISTY, Praha, Česká společnost chemická, 2003, 0009-2770.
Other information
Original language English
Type of outcome article in a journal
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.345
RIV identification code RIV/00216224:14110/03:00012312
Organization unit Faculty of Medicine
Keywords in English comparative genomics; Treponema pallidum
Tags comparative genomics, Treponema pallidum
Changed by Changed by: prof. MUDr. David Šmajs, Ph.D., učo 1116. Changed: 31/5/2005 10:12.
Abstract
DNA of Treponema pallidum subsp. pallidum Nichols strain was compared to DNA isolated from three different strains of T. pallidum subsp. pertenue (strain Gauthier, Samoan D, CDC-2). As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
Abstract (in Czech)
Byly vyšetřeny kmeny Treponema pallidum subsp. pallidum (původce syfilis) a T. pallidum subsp. pertenue (původce yaws) pomocí DNA-microarray techniky a genomového sekvenování. Zjištěné rozdíly byly diskutovány v kontextu současných poznatků.
Links
NI7351, research and development projectName: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice
Investor: Ministry of Health of the CR, Infectious Diseases, Microbiology, Epidemiology and Immunology
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