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@article{571749,
author = {Matějková, Petra and Šmajs, David and Norris, SJ and Weinstock, GM},
article_location = {Praha},
article_number = {97},
keywords = {comparative genomics; Treponema pallidum},
language = {eng},
issn = {0009-2770},
journal = {CHEMICKÉ LISTY},
title = {Comparative genomics of pathogenic Treponema pallidum subspecies},
volume = {2003},
year = {2003}
}
TY - JOUR
ID - 571749
AU - Matějková, Petra - Šmajs, David - Norris, SJ - Weinstock, GM
PY - 2003
TI - Comparative genomics of pathogenic Treponema pallidum subspecies
JF - CHEMICKÉ LISTY
VL - 2003
IS - 97
SP - 297-298
EP - 297-298
PB - Česká společnost chemická
SN - 00092770
KW - comparative genomics
KW - Treponema pallidum
N2 - DNA of Treponema pallidum subsp. pallidum Nichols strain was compared to DNA isolated from three different strains of T. pallidum subsp. pertenue (strain Gauthier, Samoan D, CDC-2). As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
ER -
MATĚJKOVÁ, Petra, David ŠMAJS, SJ NORRIS and GM WEINSTOCK. Comparative genomics of pathogenic Treponema pallidum subspecies. \textit{CHEMICKÉ LISTY}. Praha: Česká společnost chemická, 2003, vol.~2003, No~97, p.~297-298. ISSN~0009-2770.
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