ŠMAJS, David. Functional genomics as a tool to study pathogenic spirochetes. In Abstrakta XVII Biological Days - Memory in living systems. 2003. vyd. Brno: Československá biologická společnost a Biologický ústav, 2003. p. 18. ISBN 80-210-3264-2.
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Basic information
Original name Functional genomics as a tool to study pathogenic spirochetes
Name in Czech Funkční genomika jako nástroj pro studium patogenních spirochet
Name (in English) Functional genomics as a tool to study pathogenic spirochetes
Authors ŠMAJS, David (203 Czech Republic, guarantor).
Edition 2003. vyd. Brno, Abstrakta XVII Biological Days - Memory in living systems, p. 18-18, 2003.
Publisher Československá biologická společnost a Biologický ústav
Other information
Original language Czech
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/03:00012313
Organization unit Faculty of Medicine
ISBN 80-210-3264-2
Keywords in English functional genomics; pathogenic spirochetes
Tags functional genomics, pathogenic spirochetes
Changed by Changed by: prof. MUDr. David Šmajs, Ph.D., učo 1116. Changed: 31/5/2005 11:04.
Abstract
Methods of functional genomics are discussed as experimental tools to study unculturable bacterial pathogens including Treponema pallidum, the causative agent of syphilis. Functional screening of a large insert T. pallidum DNA library in E. coli using a BAC cloning system can be used for screening of gene functions governed by heterologous (T. pallidum) chromosomal DNA. T. pallidum DNA-microarray- and real-time quantitative PCR-based transcriptome analyses, utilizing organisms extracted from rabbit testicular tissue and skin lesions during infection and from bacteria isolated from time-limited in vitro tissue cultures, appear to be powerful techniques in the study of gene expression levels. Results from these studies may be useful in identifying factors that limit the multiplication of T. pallidum in vitro and thus could lead to the improvement of in vitro culture techniques. Cloning and expression of the T. pallidum proteome permits a systematic evaluation of antigenicity of T. pallidum proteins. Moreover, binding interactions using two-hybrid approaches can be examined to generate large-scale protein-protein interaction maps. Whole-genome strain comparisons performed on DNA-microarrays and XL-PCR-based genome fingerprints are used to reveal genetic differences resulting in different clinical manifestations of treponemal diseases. The genome of closely related nonpathogenic T. paraluiscuniculi is used to identify genes (missing in T. paraluiscuniculi but present in T. pallidum genome) involved in the human infections. These genes are likely to code for important virulence factors and constitute promising targets for prevention/therapy.
Abstract (in English)
Methods of functional genomics are discussed as experimental tools to study unculturable bacterial pathogens including Treponema pallidum, the causative agent of syphilis. Functional screening of a large insert T. pallidum DNA library in E. coli using a BAC cloning system can be used for screening of gene functions governed by heterologous (T. pallidum) chromosomal DNA. T. pallidum DNA-microarray- and real-time quantitative PCR-based transcriptome analyses, utilizing organisms extracted from rabbit testicular tissue and skin lesions during infection and from bacteria isolated from time-limited in vitro tissue cultures, appear to be powerful techniques in the study of gene expression levels. Results from these studies may be useful in identifying factors that limit the multiplication of T. pallidum in vitro and thus could lead to the improvement of in vitro culture techniques. Cloning and expression of the T. pallidum proteome permits a systematic evaluation of antigenicity of T. pallidum proteins. Moreover, binding interactions using two-hybrid approaches can be examined to generate large-scale protein-protein interaction maps. Whole-genome strain comparisons performed on DNA-microarrays and XL-PCR-based genome fingerprints are used to reveal genetic differences resulting in different clinical manifestations of treponemal diseases. The genome of closely related nonpathogenic T. paraluiscuniculi is used to identify genes (missing in T. paraluiscuniculi but present in T. pallidum genome) involved in the human infections. These genes are likely to code for important virulence factors and constitute promising targets for prevention/therapy.
Links
NI7351, research and development projectName: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice
Investor: Ministry of Health of the CR, Infectious Diseases, Microbiology, Epidemiology and Immunology
PrintDisplayed: 29/3/2020 12:40