KLEJDUS, Bořivoj, Radka MIKELOVÁ, Vojtěch ADAM, Josef ZEHNÁLEK, Jan VACEK, René KIZEK and Vlastimil KUBÁŇ. Liquid chromatographic-mass spectrometric determination of genistin and daidzin in soybean food samples after accelerated solvent extraction with modified content of extraction cell. Analytica Chimica Acta. 2004, vol. 517, 1-2, p. 1-11. ISSN 0003-2670.
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Basic information
Original name Liquid chromatographic-mass spectrometric determination of genistin and daidzin in soybean food samples after accelerated solvent extraction with modified content of extraction cell.
Name in Czech HPLC/MS stanovení genistinu a daidzinu v potravinách na bázi sojových bobů
Name (in English) Liquid chromatographic-mass spectrometric determination of genistin and daidzin in soybean food samples after accelerated solvent extraction with modified content of extraction cell.
Authors KLEJDUS, Bořivoj (203 Czech Republic), Radka MIKELOVÁ (203 Czech Republic), Vojtěch ADAM (203 Czech Republic, guarantor), Josef ZEHNÁLEK (203 Czech Republic), Jan VACEK (203 Czech Republic), René KIZEK (203 Czech Republic) and Vlastimil KUBÁŇ (203 Czech Republic).
Edition Analytica Chimica Acta, 2004, 0003-2670.
Other information
Original language Czech
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.588
RIV identification code RIV/00216224:14310/04:00011143
Organization unit Faculty of Science
UT WoS 000222494200001
Keywords in English accelerated solvent extraction; daidzin; foodstuffs; genistin; high-performance liquid chromatography; isoflavones; mass spectrometry; solvent extraction; Soxhlet extraction; soybean food; ultrasonic extraction; UV spectrophotometric detection
Tags accelerated solvent extraction, daidzin, foodstuffs, genistin, high-performance liquid chromatography, Isoflavones, mass spectrometry, solvent extraction, Soxhlet extraction, soybean food, ultrasonic extraction, UV spectrophotometric detection
Changed by Changed by: prof. RNDr. Vojtěch Adam, Ph.D., učo 63820. Changed: 22/5/2009 21:59.
Abstract
A newly elaborated accelerated solvent extraction (ASE) procedure allowed more accurate (<2%) and precise (<5-8% rel.) HPLC/UV/MS determination of isoflavones in different kinds of food made from soybean (products of ecological agriculture), i.e. soy farina, soy meat granulate, soy meat laminae, soy meat bits, Protmix and EKO-B1. The on-column limits of detection (LOD) for daidzin/genistin were 1.2/1.6 fmol for the signal-to-noise ratio S/N = 3 and 1-3 fmol for daidzem, genistein, formononetin, biochanin A and ononin, respectively. Final concentrations of daidzin in soybean foods fluctuated between 30 and 60 mug/g of dry matter (DM). Significantly higher concentrations of genistin (between 60 and 130 mug/g DM) were found in the soybean foods.
Abstract (in English)
A newly elaborated accelerated solvent extraction (ASE) procedure allowed more accurate (<2%) and precise (<5-8% rel.) HPLC/UV/MS determination of isoflavones in different kinds of food made from soybean (products of ecological agriculture), i.e. soy farina, soy meat granulate, soy meat laminae, soy meat bits, Protmix and EKO-B1. The on-column limits of detection (LOD) for daidzin/genistin were 1.2/1.6 fmol for the signal-to-noise ratio S/N = 3 and 1-3 fmol for daidzem, genistein, formononetin, biochanin A and ononin, respectively. Final concentrations of daidzin in soybean foods fluctuated between 30 and 60 mug/g of dry matter (DM). Significantly higher concentrations of genistin (between 60 and 130 mug/g DM) were found in the soybean foods.
Links
MSM 432100001, plan (intention)Name: Regulace biologických a technologických procesů pro konkurenceschopné zemědělství.
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