Rapid isoflavone determination in soy plants (Glycine max) by high performance liquid chromatography coupled with diode-array detector.

MIKELOVÁ, Radka, Bořivoj KLEJDUS, Jitka PETRLOVÁ, David POTĚŠIL, Vojtěch ADAM, Marie STIBOROVÁ, Petr HODEK and René KIZEK. Rapid isoflavone determination in soy plants (Glycine max) by high performance liquid chromatography coupled with diode-array detector. In Vitamins 2004 Targeted Nutritional Therapy. Pardubice: Univerzita Pardubice, 2004, p. 181-182, 292 pp. ISBN 80-7194-644-3.

Basic information

• Original name: Rapid isoflavone determination in soy plants (Glycine max) by high performance liquid chromatography coupled with diode-array detector.
• Name in Czech: Stanovení isoflavonů u soji pomocí HPLC
• Authors: MIKELOVÁ, Radka (203 Czech Republic, guarantor), Bořivoj KLEJDUS (203 Czech Republic), Jitka PETRLOVÁ (203 Czech Republic), David POTĚŠIL (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Marie STIBOROVÁ (203 Czech Republic), Petr HODEK (203 Czech Republic) and René KIZEK (203 Czech Republic).
• Edition: Pardubice, Vitamins 2004 Targeted Nutritional Therapy. p. 181-182, 292 pp. 2004.
• Publisher: Univerzita Pardubice

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/04:00011151
• Organization unit: Faculty of Science
• ISBN: 80-7194-644-3
• Keywords in English: HPLC; isoflaovne; SPE
• Tags: HPLC, isoflaovne, SPE

Abstract

Phytoestrogens are nonsteroidal isoflavones present for example in soy foodstuffs. They have many health benefits, i.e. positively affect cardiovascular deceases and act as suppressor of reactive oxygen species. On the other hand they can also conduce to cancer reveal because their estrogen-like molecular structure. Many of the anticancer mechanisms of isoflavones were detected by in vitro procedures, including cell cultures. Several new extraction procedures, such as accelerated solvent extraction, have been developed as an improvement of the traditional procedures (soxhlet and/or ultrasonication methods) and also applied on isoflavones extraction from biological materials. High performance liquid chromatography (HPLC) with various detection methods is common for isoflavone determination. Fast column in HPLC enables shorter times of determination. The goal of our work was to apply ultra fast column on HPLC/DAD determination of isoflavones with modified accelerated solvent extraction (ASE).

Abstract (in Czech)

Phytoestrogens are nonsteroidal isoflavones present for example in soy foodstuffs. They have many health benefits, i.e. positively affect cardiovascular deceases and act as suppressor of reactive oxygen species. On the other hand they can also conduce to cancer reveal because their estrogen-like molecular structure. Many of the anticancer mechanisms of isoflavones were detected by in vitro procedures, including cell cultures. Several new extraction procedures, such as accelerated solvent extraction, have been developed as an improvement of the traditional procedures (soxhlet and/or ultrasonication methods) and also applied on isoflavones extraction from biological materials. High performance liquid chromatography (HPLC) with various detection methods is common for isoflavone determination. Fast column in HPLC enables shorter times of determination. The goal of our work was to apply ultra fast column on HPLC/DAD determination of isoflavones with modified accelerated solvent extraction (ASE).

Links

• LN00A081, research and development project: Name: Signální dráhy u rostlin