Detailed Information on Publication Record
2004
Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection.
KLEJDUS, Bořivoj, Jitka PETRLOVÁ, David POTĚŠIL, Vojtěch ADAM, Radka MIKELOVÁ et. al.Basic information
Original name
Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection.
Name in Czech
Současné stanovení vitamínů rozpustných ve vodě a v tucích metodou HPLC
Authors
KLEJDUS, Bořivoj (203 Czech Republic), Jitka PETRLOVÁ (203 Czech Republic), David POTĚŠIL (203 Czech Republic), Vojtěch ADAM (203 Czech Republic, guarantor), Radka MIKELOVÁ (203 Czech Republic), Jan VACEK (203 Czech Republic), René KIZEK (203 Czech Republic) and Vlastimil KUBÁŇ (203 Czech Republic)
Edition
Analytica Chimica Acta, 2004, 0003-2670
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.588
RIV identification code
RIV/00216224:14310/04:00011152
Organization unit
Faculty of Science
UT WoS
000223567500007
Keywords in English
diode array detection; drugs; fat-soluble vitamins; high-performance liquid chromatography; mass spectrometry; pharmaceutical preparations; simultaneous determination; vitamins; water-soluble vitamins
Tags
Změněno: 22/5/2009 22:04, prof. RNDr. Vojtěch Adam, Ph.D.
V originále
Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values.
In Czech
Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values.
Links
| MSM 432100001, plan (intention) |
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