Detailed Information on Publication Record
2004
Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium.
KLEJDUS, Bořivoj, Josef ZEHNÁLEK, Vojtěch ADAM, Jiří PETŘEK, René KIZEK et. al.Basic information
Original name
Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium.
Name in Czech
HPLC/MS stanovení subpikomolárních koncentrací glutathionu v kukuřičných zrnech exponovaných kadmiu.
Authors
KLEJDUS, Bořivoj (203 Czech Republic), Josef ZEHNÁLEK (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Jiří PETŘEK (203 Czech Republic), René KIZEK (203 Czech Republic), Jan VACEK (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor), Roland ROZIK (703 Slovakia), Ladislav HAVEL (203 Czech Republic) and Vlastimil KUBÁŇ (203 Czech Republic)
Edition
Analytica Chimica Acta, 2004, 0003-2670
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10404 Polymer science
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.588
RIV identification code
RIV/00216224:14310/04:00011157
Organization unit
Faculty of Science
UT WoS
000223567500013
Keywords in English
glutathione; HPLC/MS; voltammetry; Zea mays L.
Tags
Změněno: 20/6/2008 12:09, prof. RNDr. Libuše Trnková, CSc.
V originále
Changes in fresh weight, overall protein amounts (Bradford method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for five days at three different cadmium concentrations (0, 10 and 100 uM CdCl2). A highly sensitive HPLC/MS for determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column (150 mm 2.1 mm, 3 um particle size) in an isocratic mode with acetonitrile - 0.01% TFA (5:95, flow rate 0.1 mL/min) was applied. Cadmium concentration was measured by differential pulse adsorptive stripping voltammetry (DPASV) after deposition on HMDE at potential --0.7 V (accumulation time 180 s, acetate buffer of pH 3.6) using an AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of a hanging mercury drop electrode (HMDE) as a working electrode with area 0.4 mm2, an Ag/AgCl/3 M KCl as a reference electrode and a Pt-wire as an auxiliary electrode. Maize kernels exposed to the highest cadmium concentration (100 uM) germinated formerly and much better. Rapid increase of fresh weight probably relates with more intensive uptake of water in order to decrease cadmium concentration. An intensive preservation of homeostasis of Cd ions in germinating plants by defending mechanisms might explain differences of uptake rate of cadmium. The linear increase of GSH content with exposure time at all studied concentration suggests the defending mechanisms might be triggered by concentrations of a heavy metal.
In Czech
Changes in fresh weight, overall protein amounts (Bradford method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for five days at three different cadmium concentrations (0, 10 and 100 uM CdCl2). A highly sensitive HPLC/MS for determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column (150 mm 2.1 mm, 3 um particle size) in an isocratic mode with acetonitrile - 0.01% TFA (5:95, flow rate 0.1 mL/min) was applied. Cadmium concentration was measured by differential pulse adsorptive stripping voltammetry (DPASV) after deposition on HMDE at potential --0.7 V (accumulation time 180 s, acetate buffer of pH 3.6) using an AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of a hanging mercury drop electrode (HMDE) as a working electrode with area 0.4 mm2, an Ag/AgCl/3 M KCl as a reference electrode and a Pt-wire as an auxiliary electrode. Maize kernels exposed to the highest cadmium concentration (100 uM) germinated formerly and much better. Rapid increase of fresh weight probably relates with more intensive uptake of water in order to decrease cadmium concentration. An intensive preservation of homeostasis of Cd ions in germinating plants by defending mechanisms might explain differences of uptake rate of cadmium. The linear increase of GSH content with exposure time at all studied concentration suggests the defending mechanisms might be triggered by concentrations of a heavy metal.
Links
| LN00A081, research and development project |
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| MSM 432100001, plan (intention) |
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