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@article{572252,
author = {Švec, Pavel and Štegnerová, Hana and Durnová, Eva and Sedláček, Ivo},
article_location = {Praha},
article_number = {6},
keywords = {Pseudomonas fluorescens; ribotyping; FAME; multiple enzyme restriction fragment length polymorphism; water; taxonomy},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Characterization of esculin-positive Pseudomonas fluorescens strains isolated from an underground brook},
volume = {49},
year = {2004}
}
TY - JOUR
ID - 572252
AU - Švec, Pavel - Štegnerová, Hana - Durnová, Eva - Sedláček, Ivo
PY - 2004
TI - Characterization of esculin-positive Pseudomonas fluorescens strains isolated from an underground brook
JF - Folia Microbiologica
VL - 49
IS - 6
SP - 725-730
EP - 725-730
SN - 00155632
KW - Pseudomonas fluorescens
KW - ribotyping
KW - FAME
KW - multiple enzyme restriction fragment length polymorphism
KW - water
KW - taxonomy
N2 - A group of sixteen esculin-positive fluorescent pseudomonads isolated from an underground brook flowing through a cave complex was characterized by biotyping, multiple enzyme restriction fragment length polymorphism analysis of 16S rDNA (MERFLP), ribotyping and whole cell fatty acid methyl esters analysis (FAME). All strains were phenotypically close to Pseudomonas fluorescens, but they revealed high biochemical variability as well as some reactions atypical for P. fluorescens species. Because identification of pseudomonads by the use of biochemical testing is often unclear, further techniques were applied to this group. Fingerprints obtained by MERFLP clearly showed that all strains represent P. fluorescens species. Ribotyping separated the strains analysed into four groups corresponding almost completely (with the exception of one strain) to clustering based on biochemical profiles. FAME analysis grouped all the strains into one cluster together with the P. putida (biotype A, B), P. chlororaphis and P. fluorescens biotype F representatives, but differentiated them from other FAME profiles of all pseudomonads included in the standard library TSBA 40 provided by MIDI, Inc.
ER -
ŠVEC, Pavel, Hana ŠTEGNEROVÁ, Eva DURNOVÁ a Ivo SEDLÁČEK. Characterization of esculin-positive Pseudomonas fluorescens strains isolated from an underground brook. \textit{Folia Microbiologica}. Praha, 2004, roč.~49, č.~6, s.~725-730. ISSN~0015-5632.
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