Analytical HPLC Separation of Polycyclic Aromatic Nitrogen Heterocycles.

ŠVÁBENSKÝ, Radim, Zdeněk ŠIMEK and Kamila KOČÍ. Analytical HPLC Separation of Polycyclic Aromatic Nitrogen Heterocycles. In The Raison dEtre of environmental toxicology and chemistry, SETAC Europe 15th Annual Meeting, 22-26 May 2005. Lille, France: Society of Environmental Toxicology and Chemistry, 2005, p. 198-198.

Basic information

• Original name: Analytical HPLC Separation of Polycyclic Aromatic Nitrogen Heterocycles.
• Name in Czech: Analytická separace polycyklických dusíkatých aromatických uhlovodíků
• Authors: ŠVÁBENSKÝ, Radim (203 Czech Republic), Zdeněk ŠIMEK (203 Czech Republic, guarantor) and Kamila KOČÍ (203 Czech Republic).
• Edition: Lille, France, The Raison dEtre of environmental toxicology and chemistry, SETAC Europe 15th Annual Meeting, 22-26 May 2005, p. 198-198, 1 pp. 2005.
• Publisher: Society of Environmental Toxicology and Chemistry

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/05:00013814
• Organization unit: Faculty of Science
• Keywords in English: HPLC; Polycyclic Aromatic Nitrogen Heterocycles; PANH
• Tags: HPLC, PANH, Polycyclic Aromatic Nitrogen Heterocycles

Abstract

Potential of high performance liquid chromatography for separation and determination of polycyclic aromatic nitrogen heterocycles (PANHs) is discussed. PANHs are widely spread in the environment. They are formed and released into the environment from similar sources like other polycyclic aromatic compounds. The possibility of determination of PANHs in a mixture with PAHs on octadecyl stationary phase is demonstrated. Retention behaviour of the mixture of PANHs was studied at different chromatographic conditions. Mixture of buffer/organic modifier was used as mobile phase in isocratic and gradient mode. Effect of different pH mobile phase in acidic and neutral region on retention of individual PANHs has been investigated to describe retention changes of PANHs as a function of acid-base properties. Different concentration of buffer as a component of mobile phase was used for ionic strength effect study. Different gradients of mobile phase components were investigated. The large variability in mobile phase composition allows controlling properties of HPLC determination of PANHs in particular environmental samples. Limits of detection for two detection techniques, UV-DAD and FD are compared with other methods of PANHs determination.

Abstract (in Czech)

Jsou diskutovány možnosti využití HPLC pro separaci a stanovení polycyklických aromatických dusíkatých heterocyklů ve směsi s PAH na reversní stacionární fázi. Bylo studováno retenční chování PANH v různých podmínkách mobilní fáze pufr/organický modifikátor i isokratickém i gradientovém módu. Byl sledován vliv pH mobilní fáze a iontové síly na retence PANH. Velká variabilita složení mobilní fáze umožňuje řídit podmínky HPLC stanovení PANH v konkrétních vzorcích životního prostředí. Limity detekce pro UV detektor a FLD jsou srovnatelné s LOD jiných metod použitých pro stanovení PANH.

Links

• MSM 141100003, plan (intention): Name: Zevní prostředí - karcinogeneze - onkologie

Investor: Ministry of Education, Youth and Sports of the CR, Environment - carcinogenesis - oncology

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)