Reversed Phase HPLC Separation of Polycyclic Aromatic Nitrogen Heterocycles controlled by mobile phase pH

ŠVÁBENSKÝ, Radim, Zdeněk ŠIMEK and Kamila KOČÍ. Reversed Phase HPLC Separation of Polycyclic Aromatic Nitrogen Heterocycles controlled by mobile phase pH. In Abstract Book- 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques. Stockholm, Sweden: 29th International Symposium on High Performance Liquid Phase Separations ond Related Techniques, 2005, p. 658-658.

Basic information

• Original name: Reversed Phase HPLC Separation of Polycyclic Aromatic Nitrogen Heterocycles controlled by mobile phase pH
• Name in Czech: Separace polycyklyckých aromatických uhlovodíků kontrolovaná pomocí pH mobilní fáze
• Authors: ŠVÁBENSKÝ, Radim (203 Czech Republic), Zdeněk ŠIMEK (203 Czech Republic, guarantor) and Kamila KOČÍ (203 Czech Republic).
• Edition: Stockholm, Sweden, Abstract Book- 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques, p. 658-658, 1 pp. 2005.
• Publisher: 29th International Symposium on High Performance Liquid Phase Separations ond Related Techniques

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/05:00013815
• Organization unit: Faculty of Science
• Keywords in English: HPLC ; Polycyclic Aromatic Nitrogen Heterocycles; PANH
• Tags: HPLC, PANH, Polycyclic Aromatic Nitrogen Heterocycles

Abstract

Retention behaviour of individual polycyclic aromatic nitrogen heterocycles (PANHs) was studied at different chromatographic conditions. Mixture of phosphate buffer/organic modifier was used as mobile phase in isocratic and gradient mode. Effect of different pH mobile phase on retention of individual PANHs has been investigated to describe retention changes of PANHs as a function of acid-base properties. Different buffer concentration as a component of mobile phase was used for ionic strength effect study. PANHs are widely spread in the environment. They are formed and released into the environment from similar sources like other toxic polycyclic aromatic compounds [1]. The possibility of determination of PANHs in a mixture with polycyclic aromatic hydrocarbons (PAHs) on octadecyl stationary phase without pre-separation steps is demonstrated. The large variability in mobile phase composition allows controlling properties of HPLC separation and determination of PANHs in particular environmental samples.

Abstract (in Czech)

Retention behaviour of individual polycyclic aromatic nitrogen heterocycles (PANHs) was studied at different chromatographic conditions. Mixture of phosphate buffer/organic modifier was used as mobile phase in isocratic and gradient mode. Effect of different pH mobile phase on retention of individual PANHs has been investigated to describe retention changes of PANHs as a function of acid-base properties. Different buffer concentration as a component of mobile phase was used for ionic strength effect study. PANHs are widely spread in the environment. They are formed and released into the environment from similar sources like other toxic polycyclic aromatic compounds [1]. The possibility of determination of PANHs in a mixture with polycyclic aromatic hydrocarbons (PAHs) on octadecyl stationary phase without pre-separation steps is demonstrated. The large variability in mobile phase composition allows controlling properties of HPLC separation and determination of PANHs in particular environmental samples.

Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)