D 2005

Reversed Phase HPLC Separation of Polycyclic Aromatic Nitrogen Heterocycles controlled by mobile phase pH

ŠVÁBENSKÝ, Radim, Zdeněk ŠIMEK and Kamila KOČÍ

Basic information

Original name

Reversed Phase HPLC Separation of Polycyclic Aromatic Nitrogen Heterocycles controlled by mobile phase pH

Name in Czech

Separace polycyklyckých aromatických uhlovodíků kontrolovaná pomocí pH mobilní fáze

Authors

ŠVÁBENSKÝ, Radim (203 Czech Republic), Zdeněk ŠIMEK (203 Czech Republic, guarantor) and Kamila KOČÍ (203 Czech Republic)

Edition

Stockholm, Sweden, Abstract Book- 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques, p. 658-658, 1 pp. 2005

Publisher

29th International Symposium on High Performance Liquid Phase Separations ond Related Techniques

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/05:00013815

Organization unit

Faculty of Science

Keywords in English

HPLC ; Polycyclic Aromatic Nitrogen Heterocycles; PANH
Změněno: 31/1/2006 12:31, doc. RNDr. Zdeněk Šimek, CSc.

Abstract

V originále

Retention behaviour of individual polycyclic aromatic nitrogen heterocycles (PANHs) was studied at different chromatographic conditions. Mixture of phosphate buffer/organic modifier was used as mobile phase in isocratic and gradient mode. Effect of different pH mobile phase on retention of individual PANHs has been investigated to describe retention changes of PANHs as a function of acid-base properties. Different buffer concentration as a component of mobile phase was used for ionic strength effect study. PANHs are widely spread in the environment. They are formed and released into the environment from similar sources like other toxic polycyclic aromatic compounds [1]. The possibility of determination of PANHs in a mixture with polycyclic aromatic hydrocarbons (PAHs) on octadecyl stationary phase without pre-separation steps is demonstrated. The large variability in mobile phase composition allows controlling properties of HPLC separation and determination of PANHs in particular environmental samples.

In Czech

Retention behaviour of individual polycyclic aromatic nitrogen heterocycles (PANHs) was studied at different chromatographic conditions. Mixture of phosphate buffer/organic modifier was used as mobile phase in isocratic and gradient mode. Effect of different pH mobile phase on retention of individual PANHs has been investigated to describe retention changes of PANHs as a function of acid-base properties. Different buffer concentration as a component of mobile phase was used for ionic strength effect study. PANHs are widely spread in the environment. They are formed and released into the environment from similar sources like other toxic polycyclic aromatic compounds [1]. The possibility of determination of PANHs in a mixture with polycyclic aromatic hydrocarbons (PAHs) on octadecyl stationary phase without pre-separation steps is demonstrated. The large variability in mobile phase composition allows controlling properties of HPLC separation and determination of PANHs in particular environmental samples.

Links

MSM0021622412, plan (intention)
Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)