In vitro testing of vancomycin - gentamicin loaded bone cement
to prevent prosthetic joint infection

J 2005

In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection

GALLO, Jiří, Milan KOLÁŘ, Anthony V. FLORSCHÜTZ, Radek NOVOTNÝ, Roman PANTŮČEK et. al.

Basic information

Original name

In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection

Name in Czech

Testování kostního cementu plněného vankomycinem-gentamicinem pro prevenci kloubních protetických infekcí

Authors

GALLO, Jiří (203 Czech Republic), Milan KOLÁŘ (203 Czech Republic), Anthony V. FLORSCHÜTZ (840 United States of America), Radek NOVOTNÝ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, guarantor) and Michaela KESSELOVÁ (703 Slovakia)

Edition

Biomedical Papers, Olomouc, Palacký University, 2005, 1213-8118

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

[Full Text] URL

RIV identification code

RIV/00216224:14310/05:00013848

Organization unit

Faculty of Science

Keywords in English

Prosthetic joint infection; Polymethylmethacrylate; Antibiotic loaded cement; Vancomycin;Gentamicin; Staphylococcus aureus; Staphylococcus epidermidis

Abstract

ORIG CZ

V originále

Sepsis is a greatly feared complication of total joint arthroplasty. One key question is how to prevent periopera-tive bacterial adherence, and therefore the potential for infectious complications. The objective of our study was to appraise the emerging capacity of staphylococcal survival on prosthetic materials and to analyze the in vitro eects of gentamicin and vancomycin loaded polymethylmethacrylate (PMMA) cement on bacterial adherence and growth. Hospital acquired staphylococcal strains were systematically inoculated on four orthopedic materials (ultrahigh mo-lecular weight polyethylene, PMMA without antibiotic, commercially produced PMMA loaded with gentamicin, and manually mixed PMMA loaded with gentamicin and vancomycin). Staphylococci were identied using culture and biochemical tests. The inoculated material was allowed to incubate in a liquid broth growth media and subsequently prepared for scanning electron microscopy and bacterial growth quantication. Materials without antibiotics showed evidence of staphylococcal growth. PMMA loaded with only gentamicin grew methicillin-resistant Staphylococcus aureus. Gentamicin-vancomycin loaded PMMA completely inhibited any bacterial growth.Low-dose gentamicin-vancomycin loaded PMMA prevents staphylococcal colonization better than commercially manufactured PMMA loaded with gentamicin. We recommend this combination in high-risk procedures and revision surgeries requiring bone cement.

In Czech

neuvedeno

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

GALLO, Jiří, Milan KOLÁŘ, Anthony V. FLORSCHÜTZ, Radek NOVOTNÝ, Roman PANTŮČEK and Michaela KESSELOVÁ. In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection. Biomedical Papers. Olomouc: Palacký University, 2005, vol. 149, No 1, p. 153-158. ISSN 1213-8118.

@article{573853,
   author = {Gallo, Jiří and Kolář, Milan and Florschütz, Anthony V. and Novotný, Radek and Pantůček, Roman and Kesselová, Michaela},
   article_location = {Olomouc},
   article_number = {1},
   keywords = {Prosthetic joint infection; Polymethylmethacrylate; Antibiotic loaded cement; Vancomycin;Gentamicin; Staphylococcus aureus; Staphylococcus epidermidis},
   language = {eng},
   issn = {1213-8118},
   journal = {Biomedical Papers},
   title = {In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection},
   url = {http://publib.upol.cz/~obd/fulltext/Biomed/2005/1/153.pdf},
   volume = {149},
   year = {2005}
}

TY  - JOUR
ID  - 573853
AU  - Gallo, Jiří - Kolář, Milan - Florschütz, Anthony V. - Novotný, Radek - Pantůček, Roman - Kesselová, Michaela
PY  - 2005
TI  - In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection
JF  - Biomedical Papers
VL  - 149
IS  - 1
SP  - 153-158
EP  - 153-158
PB  - Palacký University
SN  - 12138118
KW  - Prosthetic joint infection
KW  - Polymethylmethacrylate
KW  - Antibiotic loaded cement
KW  - Vancomycin;Gentamicin
KW  - Staphylococcus aureus
KW  - Staphylococcus epidermidis
UR  - http://publib.upol.cz/~obd/fulltext/Biomed/2005/1/153.pdf
N2  - Sepsis is a greatly feared complication of total joint arthroplasty. One key question is how to prevent periopera-tive bacterial adherence, and therefore the potential for infectious complications. The objective of our study was to appraise the emerging capacity of staphylococcal survival on prosthetic materials and to analyze the in vitro eects of gentamicin and vancomycin loaded polymethylmethacrylate (PMMA) cement on bacterial adherence and growth. Hospital acquired staphylococcal strains were systematically inoculated on four orthopedic materials (ultrahigh mo-lecular weight polyethylene, PMMA without antibiotic, commercially produced PMMA loaded with gentamicin, and manually mixed PMMA loaded with gentamicin and vancomycin). Staphylococci were identied using culture and biochemical tests. The inoculated material was allowed to incubate in a liquid broth growth media and subsequently prepared for scanning electron microscopy and bacterial growth quantication. Materials without antibiotics showed evidence of staphylococcal growth. PMMA loaded with only gentamicin grew methicillin-resistant Staphylococcus aureus. Gentamicin-vancomycin loaded PMMA completely inhibited any bacterial growth.Low-dose gentamicin-vancomycin loaded PMMA prevents staphylococcal colonization better than commercially manufactured PMMA loaded with gentamicin. We recommend this combination in high-risk procedures and revision surgeries requiring bone cement.
ER  -

GALLO, Jiří, Milan KOLÁŘ, Anthony V. FLORSCHÜTZ, Radek NOVOTNÝ, Roman PANTŮČEK and Michaela KESSELOVÁ. In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection. \textit{Biomedical Papers}. Olomouc: Palacký University, 2005, vol.~149, No~1, p.~153-158. ISSN~1213-8118.

Detailed Information on Publication Record