J 2005

Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods

ŠIŠKOVÁ, Zdeňka, Zdeněk GLATZ and Vladislav KAHLE

Basic information

Original name

Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods

Name in Czech

Sledování aktivity glutathion-S-trensferasy vůči styrenoxidu pomocí elektromigračních metod

Authors

ŠIŠKOVÁ, Zdeňka (203 Czech Republic), Zdeněk GLATZ (203 Czech Republic, guarantor) and Vladislav KAHLE (203 Czech Republic)

Edition

Journal of Separation Science, Weinheim, WILEY-VCH Verlag GmbH & Co. 2005, 1615-9306

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.829

RIV identification code

RIV/00216224:14310/05:00012561

Organization unit

Faculty of Science

UT WoS

000231513900012

Keywords in English

styrene oxide; GST; MEKC; CEC

Tags

Změněno: 19/5/2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Abstract

ORIG CZ

V originále

A new method has been developed for the monitoring of glutathione S-tranferase (GST) detoxification activity toward styrene oxide (SO). The enzymatic reaction was carried out directly in thermostatted autosampler vial and the formation of conjugates between glutathione (GSH) and SO was monitored by sequential MEKC runs. The determinations were performed in a 50 um fused silica capillary using 50 mM SDS in 20 mM phosphate 20 mM tetraborate buffer (pH 8.3) as a background electrolyte; separation voltage 28 kV (positive polarity), temperature of capillary 25 oC and detection at 200 nm. The method is rapid, able to be automated, and requires only small amount of samples, which is especially important in the case of GST isoenzyme analyses.

In Czech

Byla vypracována metoda pro sledování aktivity glutathion-S-trensferasy vůči styrenoxidu pomocí elektromigračních metod

Links

GA203/03/1125, research and development project
Name: Využití kapilární zónové elektroforézy pro studium enzymů
Investor: Czech Science Foundation, Application of capillary zone electrophoresis for the study of enzymes
MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment