ŠIŠKOVÁ, Zdeňka, Zdeněk GLATZ and Vladislav KAHLE. Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods. Journal of Separation Science. Weinheim: WILEY-VCH Verlag GmbH & Co., 2005, vol. 28, No 12, p. 1357-1362. ISSN 1615-9306. |
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@article{573912, author = {Šišková, Zdeňka and Glatz, Zdeněk and Kahle, Vladislav}, article_location = {Weinheim}, article_number = {12}, keywords = {styrene oxide; GST; MEKC; CEC}, language = {eng}, issn = {1615-9306}, journal = {Journal of Separation Science}, title = {Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods}, volume = {28}, year = {2005} }
TY - JOUR ID - 573912 AU - Šišková, Zdeňka - Glatz, Zdeněk - Kahle, Vladislav PY - 2005 TI - Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods JF - Journal of Separation Science VL - 28 IS - 12 SP - 1357-1362 EP - 1357-1362 PB - WILEY-VCH Verlag GmbH & Co. SN - 16159306 KW - styrene oxide KW - GST KW - MEKC KW - CEC N2 - A new method has been developed for the monitoring of glutathione S-tranferase (GST) detoxification activity toward styrene oxide (SO). The enzymatic reaction was carried out directly in thermostatted autosampler vial and the formation of conjugates between glutathione (GSH) and SO was monitored by sequential MEKC runs. The determinations were performed in a 50 um fused silica capillary using 50 mM SDS in 20 mM phosphate 20 mM tetraborate buffer (pH 8.3) as a background electrolyte; separation voltage 28 kV (positive polarity), temperature of capillary 25 oC and detection at 200 nm. The method is rapid, able to be automated, and requires only small amount of samples, which is especially important in the case of GST isoenzyme analyses. ER -
ŠIŠKOVÁ, Zdeňka, Zdeněk GLATZ and Vladislav KAHLE. Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods. \textit{Journal of Separation Science}. Weinheim: WILEY-VCH Verlag GmbH \&{} Co., 2005, vol.~28, No~12, p.~1357-1362. ISSN~1615-9306.
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