| ŠIŠKOVÁ, Zdeňka, Zdeněk GLATZ and Vladislav KAHLE. Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods. Journal of Separation Science. Weinheim: WILEY-VCH Verlag GmbH & Co., 2005, vol. 28, No 12, p. 1357-1362. ISSN 1615-9306. |
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@article{573912,
author = {Šišková, Zdeňka and Glatz, Zdeněk and Kahle, Vladislav},
article_location = {Weinheim},
article_number = {12},
keywords = {styrene oxide; GST; MEKC; CEC},
language = {eng},
issn = {1615-9306},
journal = {Journal of Separation Science},
title = {Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods},
volume = {28},
year = {2005}
}
TY - JOUR
ID - 573912
AU - Šišková, Zdeňka - Glatz, Zdeněk - Kahle, Vladislav
PY - 2005
TI - Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods
JF - Journal of Separation Science
VL - 28
IS - 12
SP - 1357-1362
EP - 1357-1362
PB - WILEY-VCH Verlag GmbH & Co.
SN - 16159306
KW - styrene oxide
KW - GST
KW - MEKC
KW - CEC
N2 - A new method has been developed for the monitoring of glutathione S-tranferase (GST) detoxification activity toward styrene oxide (SO). The enzymatic reaction was carried out directly in thermostatted autosampler vial and the formation of conjugates between glutathione (GSH) and SO was monitored by sequential MEKC runs. The determinations were performed in a 50 um fused silica capillary using 50 mM SDS in 20 mM phosphate 20 mM tetraborate buffer (pH 8.3) as a background electrolyte; separation voltage 28 kV (positive polarity), temperature of capillary 25 oC and detection at 200 nm. The method is rapid, able to be automated, and requires only small amount of samples, which is especially important in the case of GST isoenzyme analyses.
ER -
ŠIŠKOVÁ, Zdeňka, Zdeněk GLATZ and Vladislav KAHLE. Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods. \textit{Journal of Separation Science}. Weinheim: WILEY-VCH Verlag GmbH \&{} Co., 2005, vol.~28, No~12, p.~1357-1362. ISSN~1615-9306.
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