D 2005

Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese

PAPEŽOVÁ, Kateřina and Zdeněk GLATZ

Basic information

Original name

Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese

Name in Czech

Stanovení kyanidu v ul vzorcích pomocí CE a on-line reakce s rhodanasou.

Authors

PAPEŽOVÁ, Kateřina (203 Czech Republic) and Zdeněk GLATZ (203 Czech Republic, guarantor)

Edition

Stockholm, Sweden, Proceeding of 29th Symposium on HPLC and Related Techniques, p. 299-299, 1 pp. 2005

Publisher

Stocon Swedwn

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/05:00012562

Organization unit

Faculty of Science

Keywords in English

CE; Cyanide; Rhodanese

Tags

Změněno: 19/5/2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Abstract

ORIG CZ

V originále

Thirteen years ago a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed and developed, electrophoretically mediated microanalysis (EMMA). In this method the capillary is used not only as a separation medium but also as a reaction chamber. Since its discovery by Bao and Regnier [1], the EMMA methodology has been utilized in a number of biochemical systems [2,3]. In this work, the EMMA method was applied to determine cyanide after its on-capillary enzymatic reaction with rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1).Consequnetly cyanide thus could be determine as thiocyanate with adequate sensitivity by means of CE with simple UV detection. Compared to other assays, the EMMA method is rapid, can be automated, and requires only small amount of samples.

In Czech

Byla vypracována metoda pro stanovení kyanidu v ul vzorcích pomocí CE a on-line reakce s rhodanasou.

Links

GA203/03/1125, research and development project
Name: Využití kapilární zónové elektroforézy pro studium enzymů
Investor: Czech Science Foundation, Application of capillary zone electrophoresis for the study of enzymes
MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment