Další formáty:
BibTeX
LaTeX
RIS
@article{574329,
author = {Šmardová, Jana and Šmarda, Jan and Koptíková, Jana},
article_location = {Malden, USA},
article_number = {6},
keywords = {tumor suppressor; p53 mutation; p53 activity; FASAY},
language = {eng},
issn = {0301-4681},
journal = {Differentiation},
title = {Functional analysis of p53 tumor suppressor in yeast},
volume = {73},
year = {2005}
}
TY - JOUR
ID - 574329
AU - Šmardová, Jana - Šmarda, Jan - Koptíková, Jana
PY - 2005
TI - Functional analysis of p53 tumor suppressor in yeast
JF - Differentiation
VL - 73
IS - 6
SP - 261-277
EP - 261-277
PB - Blackwell Publishing
SN - 03014681
KW - tumor suppressor
KW - p53 mutation
KW - p53 activity
KW - FASAY
N2 - The p53 tumor suppressor protein is a transcription factor that mediates the cells response to various kinds of stress by preventing cell division and/or inducing apoptosis. p53 gene mutations have been detected in nearly 50% of human cancers. Several approaches have been developed for detection and analyses of p53 mutations: immunochemical methods have been developed to detect p53 protein levels; molecular analyses targeting changes in DNA structure are utilized; and functional assays are used to explore the biological properties of the p53 protein. Functional analysis of separated alleles in yeast targets the transactivation capability of the p53 protein expressed in yeast cells. This method uses p53 mRNA isolated from cells and tissues to produce p53 product by RT-PCR. This method serves as a powerful tool for distinguishing various functional types of p53 mutations. Understanding of exact impact of p53 mutation on its function is an important prerequisite for establishment of efficient anticancer therapies.
ER -
ŠMARDOVÁ, Jana, Jan ŠMARDA a Jana KOPTÍKOVÁ. Functional analysis of p53 tumor suppressor in yeast. \textit{Differentiation}. Malden, USA: Blackwell Publishing, roč.~73, č.~6, s.~261-277. ISSN~0301-4681. 2005.
|
