CREB-binding protein sensitizes v-myb-transformed monoblasts to differentiation inducers

ŠMARDA, Jan, Jana ŠMARDOVÁ, Alice NEMAJEROVÁ and Jarmila NAVRÁTILOVÁ. CREB-binding protein sensitizes v-myb-transformed monoblasts to differentiation inducers. In Functional Genomics and Disease. Oslo: European Science Foundation, 2005, p. 114-115. ISBN JB484MB001322OS.

Basic information

• Original name: CREB-binding protein sensitizes v-myb-transformed monoblasts to differentiation inducers
• Name in Czech: CBP zvyšuje citlivost monoblastů transformovaných v-myb k induktorům diferenciace
• Authors: ŠMARDA, Jan (203 Czech Republic, guarantor), Jana ŠMARDOVÁ (203 Czech Republic), Alice NEMAJEROVÁ (203 Czech Republic) and Jarmila NAVRÁTILOVÁ (203 Czech Republic).
• Edition: Oslo, Functional Genomics and Disease, p. 114-115, 2 pp. 2005.
• Publisher: European Science Foundation

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: Norway
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/05:00012609
• Organization unit: Faculty of Science
• ISBN: JB484MB001322OS
• Keywords in English: CBP; Myb; differentiation; trichostatin A; TPA
• Tags: CBP, differentiation, Myb, TPA, trichostatin A

Abstract

CREB-binding protein (CBP) regulates gene expression by binding to certain components of basal transcription machinery and by histone acetylation. In addition, it integrates various cellular signaling pathways through binding to multiple transcription factors, including the Myb proteins. We report in this study that CBP can partially suppress function of the v-Myb oncoprotein in leukemic cells. Although originally described as activator of v-Myb function, we show that CBP can also act as v-Myb suppressor. Ectopic expression of murine CBP in v-Myb-transformed chicken monoblasts reduced transcriptional activation abilities of the v-Myb protein and increased sensitivity to differentiation inducers such as phorbol ester or trichostatin A. In addition, exogenous CBP affected morphology of differentiated cells derived from BM2 monoblasts. Altogether, these results indicate that cellular context is an important factor to determine whether CBP will activate or suppress the protein it targets.

Abstract (in Czech)

CBP řídí genovou expresi tím, že váže určité složky bazálního transkripčního aparátu a acetylací histonů. Kromě toho se podílí na integraci různých signálních drah. Váže se např. na transkripční faktor Myb. V této práci popisujeme částečnou supresi onkoproteinu v-Myb proteinem CBP v leukemických buňkách. Ektopická exprese CBP v kuřecích monoblastech transformovaných v-myb snížila transkripčně aktivační schopnost proteinu v-Myb, zvýšila citlivost těchto buněk k induktorům diferenciace TPA a trichostatinu A a ovlivnila jejich morfologii. Tyto výsledky dokazují, že buněčný kontext je důležitý faktor, který rozhoduje, zda CBP bude aktivovat nebo suprimovat svůj cílový protein.

Links

• GA301/03/1055, research and development project: Name: Studium molekulárních mechanismů způsobujících supresi v-Myb s využitím přístupů proteomiky

Investor: Czech Science Foundation, Study of molecular mechanisms causing v-Myb suppression using proteomics-based approach

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations