KREJÍ, Eva, Anna ANDELOVÁ, Iva PORAZILOVÁ and Ivo SEDLÁČEK. Aeromonas hydrophila ssp. dhakensis - first occurrence in Czech Republic confirmed by ribotyping. In Abstract book. 2005.
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Basic information
Original name Aeromonas hydrophila ssp. dhakensis - first occurrence in Czech Republic confirmed by ribotyping
Name in Czech Aeromonas hydrophila ssp. dhakensis - první výskyt v České republice potvrzený ribotypizací
Authors KREJÍ, Eva (203 Czech Republic), Anna ANDELOVÁ (203 Czech Republic), Iva PORAZILOVÁ (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic, guarantor).
Edition Abstract book, 2005.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Canada
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/05:00013914
Organization unit Faculty of Science
Keywords in English Aeromonas; ribotyping; cellular fatty acid
Tags Aeromonas, cellular fatty acid, ribotyping
Changed by Changed by: prof. RNDr. Ivo Sedláček, CSc., učo 866. Changed: 31/3/2010 11:08.
Abstract
Aeromonads are a new unusual pathogens, which can cause mainly gastroenteritis as well as wound infection or septicaemia. Identification of aeromonads to the species level is due to ever changing taxonomy very difficult and sometimes doubtful. Recently, three subspecies of A. hydrophila - A. hydrophila ssp. hydrophila, A. hydrophila ssp. dhakensis and A. hydrophila ssp. ranae have been described. Aim of the study was to prove if the mentioned taxons can be isolated from clinical samples and identified in routine clinical laboratory and if also A. hydrophila ssp. dhakensis could be involved in diarrheal diseases in our region. 138 isolates of aeromonads were obtained in routine survey in 2003 and 2004. Biochemical identification was done by ENTEROtest24 (Pliva-Lachema), nevertheless the supplementary conventional biochemical tests (acid production from arabinose, salicin, arbutin, hydrolysis of aesculin) were done. Results were evaluated by software TNW (Pliva-Lachema). Cellular fatty acid analysis was performed by MIS Sherlock (MIDI, Inc., USA). Ribotyping of selected isolates and reference cultures using EcoRI restriction enzyme and DNA probe complementary to 16S and 23S rRNA was accomplished. From 138 aeromonads 35 A. hydrophila isolates were found. Biochemical identification of isolates based on commercial kit was often uncertain due to variations observed in some tests and required additional testing. Among them, only one strain from patient who probably acquired the infection in Egypt, was identified as A. hydrophila ssp. dhakensis on the base of ribotyping. All other isolates were identified as A. hydrophila ssp. hydrophila, none of A. hydrophila ssp. ranae was proved. Species identification was confirmed also by fatty acid analysis. The less frequent A. hydrophila ssp. dhakensis was occurred in our region. Use of an appropriate identification approach can easily warn microbiologist of a rare species. Diarrhoea due to A. hydrophila ssp. dhakensis was an imported case of infection and ribotyping appeared to be a good tool for the identification of aeromonads.
Abstract (in Czech)
Průkaz nového patogena A. hydrophyla ssp. dhakensis.
Links
MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
NR8011, research and development projectName: Identifikace Aeromonas spp. izolovaných z klinického materiálu
Investor: Ministry of Health of the CR, Identification of Aeromonas spp.from clinical samples
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