KREJČÍ, Eva, Anna ANDĚLOVÁ, Iva PORAZILOVÁ and Ivo SEDLÁČEK. Aeromonas spp. as a causative agent of acute diarrhoea in children. In Abstract book. 2005.
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Basic information
Original name Aeromonas spp. as a causative agent of acute diarrhoea in children
Name in Czech Aeromonády jako původci akutních průjmů u dětí
Authors KREJČÍ, Eva (203 Czech Republic), Anna ANDĚLOVÁ (203 Czech Republic), Iva PORAZILOVÁ (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic, guarantor).
Edition Abstract book, 2005.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Canada
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/05:00015212
Organization unit Faculty of Science
Keywords in English Aeromonas; diarrhoea; ribotyping; fatty acid analysis
Tags Aeromonas, diarrhoea, fatty acid analysis, ribotyping
Changed by Changed by: prof. RNDr. Ivo Sedláček, CSc., učo 866. Changed: 31/3/2010 11:09.
Abstract
Aeromonas spp. are known to do cause acute gastroenteritis. Often predisposed are also very young children. We described four cases of an acute watery diarrhoea due to aeromonads. All children were up to one year old and by these patients aeromonads were repeatedly isolated from stool samples. For two of them hospital care was needed to improve their state of health. Our findings should contribute to the knowledge, if there is any link between such diarrhoea cases and the exact Aeromonas species. Aeromonads were obtained from stool samples during a routine survey in 2003 and 2004. Isolates were identified by commercially available biochemical kit ENTEROtest24 (Pliva-Lachema), conventional biochemical testing, fatty acid analysis and ribotyping. In all used methods type strains of all so far described species were included in the study. Isolation of aeromonads were performed from two-week to six-week period. In two cases of diarrhoea we identified A. caviae species of which one was in consequence of one strain. The strain identity was validated by ribotyping. A. caviae isolates from the other case differed by production of acid from cellobiose and salicin. The probable source of the different A. caviae species infection was fresh water. From remaining two gastroenteritis were identified A. veronii bv. sobria/A. caviae and A. hydrophila ssp. hydrophila/A. caviae. In the second case a likely source of infection were grandparents of child who were suffered from diarrhoea after homecoming from abroad. In both other mentioned cases stayed the source of the infection unknown. Identification to the species level was confirmed by ribotyping and fatty acid analysis. Other enteric pathogens as Salmonella, Campylobacter and Yersinia were not present in none of cases. Four cases of small children suffering from an acute gastroenteritis proved, that aeromonads can cause serious health problems, which are not so rare. Whatever epidemiological circumstances are still remaining unclear. We did not prove that this clinically important cases were due to infection of one exact Aeromonas species.
Abstract (in Czech)
Aeromonády vystupují jako původci střevních infekcí a to především u malých dětí. Podstatou našeho sdělení je popis čtyř případů akutního vodnatého průjmu u malých dětí zapříčiněného aeromonádami, což si vyžádalo i hospitalizaci na lůžkovém oddělení. Biochemická identifikace aeromonád byla potvrzena ribotypizací. Prokázali jsme, ze aeromonádove infekce nejsou nijak vzácné, ale nebyl zjištěn žádný dominantní druh typický pro tyto případy vodnatých průjmů.
Links
NR8011, research and development projectName: Identifikace Aeromonas spp. izolovaných z klinického materiálu
Investor: Ministry of Health of the CR, Identification of Aeromonas spp.from clinical samples
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