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@inproceedings{577001, author = {Křížová, Jana and Španová, Alena and Rittich, Bohuslav}, address = {Ljubljana, Slovenia}, booktitle = {The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia}, keywords = {Bifidobacterium spp.; identification; genus and species specific PCR; ARDRA}, language = {eng}, location = {Ljubljana, Slovenia}, isbn = {961-90346-3-5}, pages = {151-151}, publisher = {Slovenian Microbiology Society}, title = {Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis}, year = {2004} }
TY - JOUR ID - 577001 AU - Křížová, Jana - Španová, Alena - Rittich, Bohuslav PY - 2004 TI - Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis PB - Slovenian Microbiology Society CY - Ljubljana, Slovenia SN - 9619034635 KW - Bifidobacterium spp. KW - identification KW - genus and species specific PCR KW - ARDRA N2 - Molecular methods were applied to verify the species identification of Bifidobacterium isolates from the Culture Collection of Dairy Microorganisms (CCDM, Tábor, ČR), previously identified by fenotypic techniques only. The methods used for identification in this study were the genus and species specific PCR and restriction analysis of amplified ribosomal DNA restriction analysis with three restriction enzymes. The methods were introduced and optimalised using type and reference strains of Bifidobacterium species. The molecular methods chosen enabled species identification of the analysed strains and provided congruent results. ER -
KŘÍŽOVÁ, Jana, Alena ŠPANOVÁ and Bohuslav RITTICH. Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis. In \textit{The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia}. Ljubljana, Slovenia: Slovenian Microbiology Society, 2004, p.~151. ISBN~961-90346-3-5.
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