KŘÍŽOVÁ, Jana, Alena ŠPANOVÁ and Bohuslav RITTICH. Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis. In The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia. Ljubljana, Slovenia: Slovenian Microbiology Society, 2004, p. 151. ISBN 961-90346-3-5.
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Basic information
Original name Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis
Name in Czech Identifikace bifidobakterií s využitím metod PCR a ARDRA
Authors KŘÍŽOVÁ, Jana (203 Czech Republic), Alena ŠPANOVÁ (203 Czech Republic) and Bohuslav RITTICH (203 Czech Republic, guarantor).
Edition Ljubljana, Slovenia, The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia, p. 151-151, 2004.
Publisher Slovenian Microbiology Society
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Slovenia
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/04:00019893
Organization unit Faculty of Science
ISBN 961-90346-3-5
Keywords in English Bifidobacterium spp.; identification; genus and species specific PCR; ARDRA
Tags ARDRA, Bifidobacterium spp., identification
Changed by Changed by: RNDr. Jana Křížová, Ph.D., učo 21413. Changed: 19/9/2005 18:12.
Abstract
Molecular methods were applied to verify the species identification of Bifidobacterium isolates from the Culture Collection of Dairy Microorganisms (CCDM, Tábor, ČR), previously identified by fenotypic techniques only. The methods used for identification in this study were the genus and species specific PCR and restriction analysis of amplified ribosomal DNA restriction analysis with three restriction enzymes. The methods were introduced and optimalised using type and reference strains of Bifidobacterium species. The molecular methods chosen enabled species identification of the analysed strains and provided congruent results.
Abstract (in Czech)
Molekulárně-biologické metody (rodově a druhově specifická PCR a restrikční analýza amplifikované 16S rDNA s využitím 3 restrikčních enzymů) byly zvoleny pro ověření správnosti fenotypové identifikace izolátů bifidobakterií ze sbírky CCDM (Tábor, ČR). Molekulární metody byly zavedeny a optimalizovány s využitím typových a referenčních kultur bifidobakterií. Zvolené techniky umožnily identifikaci CCDM izolátů na úrověň druhů a poskytovaly shodné výsledky.
Links
GA202/02/1361, research and development projectName: Využití magnetických nosičů s imobilizovanými selektivními ligandy v DNA diagnostice
Investor: Czech Science Foundation, The use of magnetic carriers with immobilized selective ligands in DNA diagnostics
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