Identification of bifidobacteria by polymerase chain reaction
and amplified ribosomal DNA restriction analysis

D 2004

Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis

KŘÍŽOVÁ, Jana, Alena ŠPANOVÁ and Bohuslav RITTICH

Basic information

Original name

Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis

Name in Czech

Identifikace bifidobakterií s využitím metod PCR a ARDRA

Authors

KŘÍŽOVÁ, Jana (203 Czech Republic), Alena ŠPANOVÁ (203 Czech Republic) and Bohuslav RITTICH (203 Czech Republic, guarantor)

Edition

Ljubljana, Slovenia, The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia, p. 151-151, 2004

Publisher

Slovenian Microbiology Society

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Slovenia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/04:00019893

Organization unit

Faculty of Science

ISBN

961-90346-3-5

Keywords in English

Bifidobacterium spp.; identification; genus and species specific PCR; ARDRA

Abstract

ORIG CZ

V originále

Molecular methods were applied to verify the species identification of Bifidobacterium isolates from the Culture Collection of Dairy Microorganisms (CCDM, Tábor, ČR), previously identified by fenotypic techniques only. The methods used for identification in this study were the genus and species specific PCR and restriction analysis of amplified ribosomal DNA restriction analysis with three restriction enzymes. The methods were introduced and optimalised using type and reference strains of Bifidobacterium species. The molecular methods chosen enabled species identification of the analysed strains and provided congruent results.

In Czech

Molekulárně-biologické metody (rodově a druhově specifická PCR a restrikční analýza amplifikované 16S rDNA s využitím 3 restrikčních enzymů) byly zvoleny pro ověření správnosti fenotypové identifikace izolátů bifidobakterií ze sbírky CCDM (Tábor, ČR). Molekulární metody byly zavedeny a optimalizovány s využitím typových a referenčních kultur bifidobakterií. Zvolené techniky umožnily identifikaci CCDM izolátů na úrověň druhů a poskytovaly shodné výsledky.

Links

GA202/02/1361, research and development project

Name: Využití magnetických nosičů s imobilizovanými selektivními ligandy v DNA diagnostice

Investor: Czech Science Foundation, The use of magnetic carriers with immobilized selective ligands in DNA diagnostics

Citovat

KŘÍŽOVÁ, Jana, Alena ŠPANOVÁ and Bohuslav RITTICH. Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis. In The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia. Ljubljana, Slovenia: Slovenian Microbiology Society, 2004, p. 151. ISBN 961-90346-3-5.

@inproceedings{577001,
   author = {Křížová, Jana and Španová, Alena and Rittich, Bohuslav},
   address = {Ljubljana, Slovenia},
   booktitle = {The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia},
   keywords = {Bifidobacterium spp.; identification; genus and species specific PCR; ARDRA},
   language = {eng},
   location = {Ljubljana, Slovenia},
   isbn = {961-90346-3-5},
   pages = {151-151},
   publisher = {Slovenian Microbiology Society},
   title = {Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis},
   year = {2004}
}

TY  - JOUR
ID  - 577001
AU  - Křížová, Jana - Španová, Alena - Rittich, Bohuslav
PY  - 2004
TI  - Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis
PB  - Slovenian Microbiology Society
CY  - Ljubljana, Slovenia
SN  - 9619034635
KW  - Bifidobacterium spp.
KW  - identification
KW  - genus and species specific PCR
KW  - ARDRA
N2  - Molecular methods were applied to verify the species identification of Bifidobacterium isolates from the Culture Collection of Dairy Microorganisms (CCDM, Tábor, ČR), previously identified by fenotypic techniques only. The methods used for identification in this study were the genus and species specific PCR and restriction analysis of amplified ribosomal DNA restriction analysis with three restriction enzymes. The methods were introduced and optimalised using type and reference strains of Bifidobacterium species. The molecular methods chosen enabled species identification of the analysed strains and provided congruent results.
ER  -

KŘÍŽOVÁ, Jana, Alena ŠPANOVÁ and Bohuslav RITTICH. Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis. In \textit{The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia}. Ljubljana, Slovenia: Slovenian Microbiology Society, 2004, p.~151. ISBN~961-90346-3-5.

Detailed Information on Publication Record