Detailed Information on Publication Record
2005
Capillary Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Native Fluorescence Detection for Protein Analysis
PREISLER, Jan, Patrik VRÁBEL, Ondřej PEŠ, Helena ŘEHULKOVÁ, Josef HAVEL et. al.Basic information
Original name
Capillary Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Native Fluorescence Detection for Protein Analysis
Name in Czech
Kapilární elektroforéza spojená s hmotnostní spektrometrií MALDI a nativní laserem indukovanou fluorescenční detekcí pro analýzu proteinů
Authors
PREISLER, Jan (203 Czech Republic, belonging to the institution), Patrik VRÁBEL (203 Czech Republic, belonging to the institution), Ondřej PEŠ (203 Czech Republic, belonging to the institution), Helena ŘEHULKOVÁ (203 Czech Republic, belonging to the institution) and Josef HAVEL (203 Czech Republic, guarantor, belonging to the institution)
Edition
Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied topics, 2005
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10406 Analytical chemistry
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/05:00012665
Organization unit
Faculty of Science
Keywords in English
MALDI; LIF; CE; digest
Změněno: 11/4/2013 16:35, prof. Mgr. Jan Preisler, Ph.D.
V originále
We present combination of capillary electrophoresis (CE) with fluorescence and matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) detection for protein analysis and identification. After separation in CE column, proteins are deposited on MALDI target. The position of protein bands can be found using native fluorescence excited with a 266 nm laser. The proteins in the bands of interest are digested directly on MALDI target and, after addition of MALDI matrix, identified from peptide fingerprint using MALDI MS. Addition of matrix after separation enables true decoupling and independent optimization of CE and MALDI MS. The presented arrangement is an alternative approach to commonly used methods, such as combination of gel electrophoresis and MALDI MS.
In Czech
V této práci je prezentováno spojení kapilární elektroforézy (CE) s fluorescenční a MALDI MS detekcí pro analýzu proteinů a jejich identifikaci.
Links
| GA203/03/0515, research and development project |
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| MSM0021622415, plan (intention) |
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