PROTEOMIC STRATEGY FOR INVESTIGATIGATION OF CYTOKININ ACTION IN ARABIDOPSIS THALIANA

BÖHMOVÁ, Gabriela, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Šárka KOUKALOVÁ and Břetislav BRZOBOHATÝ. PROTEOMIC STRATEGY FOR INVESTIGATIGATION OF CYTOKININ ACTION IN ARABIDOPSIS THALIANA. In Molecular and cellular proteomics. Irvine: American society for biochemistry and molecular biology, 2005, p. 256. ISSN 1535-9476.

Basic information

Original name

PROTEOMIC STRATEGY FOR INVESTIGATIGATION OF CYTOKININ ACTION IN ARABIDOPSIS THALIANA

Name in Czech

Strategie pro zjištění působení cytokininů v Arabidopsis thaliana

Authors

BÖHMOVÁ, Gabriela (203 Czech Republic, guarantor), Zbyněk ZDRÁHAL (203 Czech Republic), Hana KONEČNÁ (203 Czech Republic), Šárka KOUKALOVÁ (203 Czech Republic) and Břetislav BRZOBOHATÝ (203 Czech Republic)

Edition

Irvine, Molecular and cellular proteomics, p. 256-256, 2005

Publisher

American society for biochemistry and molecular biology

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Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 9.876

RIV identification code

RIV/00216224:14310/05:00013978

Organization unit

Faculty of Science

ISSN

UT WoS

000202995300660

Keywords in English

proteomics; mass spectrometry; plant hormone

Tags

mass spectrometry, plant hormone, proteomics

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Abstract

V originále

Proteomics-based approach for the investigation of Arabidopsis thaliana seedlings with cytokinin overproduction was applied. We used both MALDI-MS and LC-MS/MS techniques for peptide analysis. Our results showed the effective application of different methods for protein identification. In our case the combination of MALDI-MS and LC-MS/MS increased the number of identified proteins to over 90 % of all analysed samples.

In Czech

Využili jsme proteomických nástrojů k výzkumu semenáčků Arabidopsis, které nadprodukují cytokininy. Pro identifikaci proteinů byly použity metody hmotnostní spektrometrie MALDI-MS a LC-MS/MS. Kombinace těchto metod umožnila zvýšit počet identifikovaných proteinů nad 90%.

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Links

MSM 143100008, plan (intention)	Name: Genomy a jejich funkce Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
MSM0021622415, plan (intention)	Name: Molekulární podstata buněčných a tkáňových regulací Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations