Detailed Information on Publication Record
2005
Molecular mapping of some Arabidopsis thaliana genes determining leaf shape and chlorophyll defects.
ŘEPKOVÁ, Jana, Sylva HLAVÁČOVÁ, Pavel LÍZAL, Zdeňka KYJOVSKÁ, Jiřina RELICHOVÁ et. al.Basic information
Original name
Molecular mapping of some Arabidopsis thaliana genes determining leaf shape and chlorophyll defects.
Name in Czech
Molekulární mapování některých genů Arabidopsis thaliana determinujících tvar listu a defekty chlorofylu
Authors
ŘEPKOVÁ, Jana (203 Czech Republic, guarantor), Sylva HLAVÁČOVÁ (203 Czech Republic), Pavel LÍZAL (203 Czech Republic), Zdeňka KYJOVSKÁ (203 Czech Republic) and Jiřina RELICHOVÁ (203 Czech Republic)
Edition
Biologia, Bratislava, Bratislava, Slovak Academy of Sciences, 2005, 1335-6372
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Slovakia
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14330/05:00012695
Organization unit
Faculty of Informatics
UT WoS
000232053000019
Keywords in English
Arabidopsis thaliana; CAPS; DNA markers; genetic mapping; SSR
Změněno: 15/1/2007 10:51, prof. RNDr. Jana Řepková, CSc.
V originále
Six Arabidopsis thaliana mutations were developed in our laboratory and are held at the Nottingham Arabidopsis Stock Centre. Morphological mutations were called cupuliformis, rotundata and involuta. The second group of mutants with chlorophyll defects were chlorominuta, lucida(S) and lucida. The aim of our work was to localise the six mutant alleles on the genetic map of A. thaliana. The level of DNA polymorphism among ecotypes of A. thaliana, S96 and Dijon-G (genetic backgrounds of mutations) vs. Columbia and Landsberg erecta, had to be evaluated to determine suitable parental plants for the crosses. DNA markers, 16 microsatellites and 6 cleaved amplified polymorphic sequences, were used for recombination analysis in F2 populations. The location of the cupuliformis mutation was on the short arm of chromosome 1, the rotundata mutation on the long arm of chromosome 4 and the involuta mutation on the long arm of chromosome 2. The chlorominuta mutation with a chlorophyll defect was located on chromosome 3 and the positions of both lucida and lucida(S) mutations were on chromosome 4. This mapping study simplified gene identification and evaluation of potential allelism with mutants already isolated by previous authors.
In Czech
V naší laboratoři bylo vyvinuto šest mutací, které jsou uchovávány v Nottingham Arabidopsis Stock Centre. Morfologické mutace byly nazvány cupuliformis, rotundata a involuta. Druhá skupina mutací s chlorofylovými defekty byly chlorominuta, lucida(S) a lucida. Byl vyhodnocen polymorfismus mezi genetickými pozadími Dijon.G, Columbia a Landberg erecta. DNA markery, 16 mikrosatelitů a 6 CAPS, byly využity k rekombinační analýze v F2 populacích. Lokalizace mutace cupuliformis byla na krátkém rameni chromozomu 1, mutace rotundata na dlouhém rameni chromozomu 4 a mutace involuta na dlouhém rameni chromozomu 2. Mutace s chlorofylovým defektem chlorominuta byla lokalizována na chromozomu 3 a obě mutace lucida a lucida(S) byly lokalizovány na chromozomu 4.
Links
| GD204/05/H505, research and development project |
| ||
| MSM0021622415, plan (intention) |
|