| ŘEPKOVÁ, Jana, Sylva HLAVÁČOVÁ, Pavel LÍZAL, Zdeňka KYJOVSKÁ and Jiřina RELICHOVÁ. Molecular mapping of some Arabidopsis thaliana genes determining leaf shape and chlorophyll defects. Biologia, Bratislava. Bratislava: Slovak Academy of Sciences, 2005, vol. 60, No 4, p. 443-449. ISSN 1335-6372. |
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@article{583262,
author = {Řepková, Jana and Hlaváčová, Sylva and Lízal, Pavel and Kyjovská, Zdeňka and Relichová, Jiřina},
article_location = {Bratislava},
article_number = {4},
keywords = {Arabidopsis thaliana; CAPS; DNA markers; genetic mapping; SSR},
language = {eng},
issn = {1335-6372},
journal = {Biologia, Bratislava},
title = {Molecular mapping of some Arabidopsis thaliana genes determining leaf shape and chlorophyll defects.},
volume = {60},
year = {2005}
}
TY - JOUR
ID - 583262
AU - Řepková, Jana - Hlaváčová, Sylva - Lízal, Pavel - Kyjovská, Zdeňka - Relichová, Jiřina
PY - 2005
TI - Molecular mapping of some Arabidopsis thaliana genes determining leaf shape and chlorophyll defects.
JF - Biologia, Bratislava
VL - 60
IS - 4
SP - 443-449
EP - 443-449
PB - Slovak Academy of Sciences
SN - 13356372
KW - Arabidopsis thaliana
KW - CAPS
KW - DNA markers
KW - genetic mapping
KW - SSR
N2 - Six Arabidopsis thaliana mutations were developed in our laboratory and are held at the Nottingham Arabidopsis Stock Centre. Morphological mutations were called cupuliformis, rotundata and involuta. The second group of mutants with chlorophyll defects were chlorominuta, lucida(S) and lucida. The aim of our work was to localise the six mutant alleles on the genetic map of A. thaliana. The level of DNA polymorphism among ecotypes of A. thaliana, S96 and Dijon-G (genetic backgrounds of mutations) vs. Columbia and Landsberg erecta, had to be evaluated to determine suitable parental plants for the crosses. DNA markers, 16 microsatellites and 6 cleaved amplified polymorphic sequences, were used for recombination analysis in F2 populations. The location of the cupuliformis mutation was on the short arm of chromosome 1, the rotundata mutation on the long arm of chromosome 4 and the involuta mutation on the long arm of chromosome 2. The chlorominuta mutation with a chlorophyll defect was located on chromosome 3 and the positions of both lucida and lucida(S) mutations were on chromosome 4. This mapping study simplified gene identification and evaluation of potential allelism with mutants already isolated by previous authors.
ER -
ŘEPKOVÁ, Jana, Sylva HLAVÁČOVÁ, Pavel LÍZAL, Zdeňka KYJOVSKÁ and Jiřina RELICHOVÁ. Molecular mapping of some Arabidopsis thaliana genes determining leaf shape and chlorophyll defects. \textit{Biologia, Bratislava}. Bratislava: Slovak Academy of Sciences, 2005, vol.~60, No~4, p.~443-449. ISSN~1335-6372.
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