Detailed Information on Publication Record
2005
Study of Derivatization of Peptides and Proteins for Fluorescence Detection Using Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
VRÁBEL, Patrik, Jan PREISLER, Markéta RYVOLOVÁ, Helena ŘEHULKOVÁ, Petr TÁBORSKÝ et. al.Basic information
Original name
Study of Derivatization of Peptides and Proteins for Fluorescence Detection Using Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Name in Czech
Studium derivatizace peptidů a proteinů pro fluorescenční detekci pomocí kapilární elektroforézy a hmotnostní spektrometrie s laserovou desorpcí a ionizací
Authors
VRÁBEL, Patrik (203 Czech Republic, guarantor, belonging to the institution), Jan PREISLER (203 Czech Republic, belonging to the institution), Markéta RYVOLOVÁ (203 Czech Republic, belonging to the institution), Helena ŘEHULKOVÁ (203 Czech Republic, belonging to the institution) and Petr TÁBORSKÝ (203 Czech Republic, belonging to the institution)
Edition
Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied topics, 2005
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10406 Analytical chemistry
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/05:00012705
Organization unit
Faculty of Science
Keywords in English
Derivatization; Electrophoresis Capillary; MALDI; Mass Spectrometry; Capillary Electrophoresis; Proteomic;
Tags
Změněno: 9/4/2013 14:23, prof. Mgr. Jan Preisler, Ph.D.
V originále
We presented an effective combination of two analytical techniques (CE-MALDI MS) for sensitive identification of fluorescently labeled polypeptides and optimization of derivatization procedure. In analysis of compounds, which included derivatization step, the detection was found to be limited by chemistry and separation rather than by the instrument performance. This was confirmed on CE-LIF of peptides and proteins derivatized with rhodamine B isothiocyanate (RBITC). Identification of all derivatized species was not possible with fluorescence detection; therefore CE experiments with mass spectrometric detection of derivatization products were applied. Optimized CE separation of peptides and small proteins was coupled to MALDI MS; common MALDI matrices were used. This experimental work confirmed suitability of MALDI MS for detection and identification of peptides and proteins derivatized with rhodamine-based dyes. The information from the experiment will be used for optimizing of the derivatization step.
In Czech
Charakterizace kombinace CE - MALDI MS a derivatizace peptidů a menších proteinů fluorescenčním reagentem.
Links
| GA203/03/0515, research and development project |
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| MSM0021622415, plan (intention) |
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