Detailed Information on Publication Record
2005
Addressing the role of vimentin in monocyte/macrophage differentiation using RNAi approach
MACEČKOVÁ, Vendula, Petr BENEŠ and Jan ŠMARDABasic information
Original name
Addressing the role of vimentin in monocyte/macrophage differentiation using RNAi approach
Name in Czech
Výzkum úlohy vimentinu v monocytové/makrofágové diferenciační dráze založený na přístupu RNAi
Name (in English)
Addressing the role of vimentin in monocyte/macrophage differentiation using RNAi approach
Authors
MACEČKOVÁ, Vendula (203 Czech Republic), Petr BENEŠ (203 Czech Republic) and Jan ŠMARDA (203 Czech Republic, guarantor)
Edition
České Budějovice, RNA club, p. 9-9, 2005
Publisher
Přírodovědecká fakulta, Univerzita Karlova v Praze
Other information
Language
Czech
Type of outcome
Stať ve sborníku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/05:00012709
Organization unit
Faculty of Science
ISBN
1214-8598
Keywords in English
vimentin; differentiation; RNAi; phagocytosis
Tags
Změněno: 21/12/2006 11:54, prof. RNDr. Jan Šmarda, CSc.
V originále
Vimentin is one of the IF proteins that undergo significant changes both in expression, structure and subcellular location during maturation of hematopoietic cells. The v-myb-transformed monoblasts of the cell line BM2 can be induced to differentiate towards macrophage-like cells using phorbol ester TPA and histon deacetylase inhibitor trichostatin A (TSA). We described earlier that level of vimentin increases during TPA- or TSA-induced differentiation of BM2 cells. In this study, RNAi technology was used to explore the role of vimentin in differentiation of BM2 cells. The cells were transfected with vector expressing siRNA targeted to endogenous vimentin mRNA and several independ clones of stable transfectants exhibiting 70% reduction of cellular vimentin level were obtained. The lack of vimentin altered morphology of the cells, reduced their phagocytic activity and production of ROS upon treatment with TPA or TSA. Our results demonstrate that up-regulation of vimentin is essential for formation of fully active macrophages during terminal steps of monocyte/macrophage differentiation.
In English
Vimentin is one of the IF proteins that undergo significant changes both in expression, structure and subcellular location during maturation of hematopoietic cells. The v-myb-transformed monoblasts of the cell line BM2 can be induced to differentiate towards macrophage-like cells using phorbol ester TPA and histon deacetylase inhibitor trichostatin A (TSA). We described earlier that level of vimentin increases during TPA- or TSA-induced differentiation of BM2 cells. In this study, RNAi technology was used to explore the role of vimentin in differentiation of BM2 cells. The cells were transfected with vector expressing siRNA targeted to endogenous vimentin mRNA and several independ clones of stable transfectants exhibiting 70% reduction of cellular vimentin level were obtained. The lack of vimentin altered morphology of the cells, reduced their phagocytic activity and production of ROS upon treatment with TPA or TSA. Our results demonstrate that up-regulation of vimentin is essential for formation of fully active macrophages during terminal steps of monocyte/macrophage differentiation.
Links
| GA301/03/1055, research and development project |
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| GP301/03/D022, research and development project |
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| MSM0021622415, plan (intention) |
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