ŠVEC, Pavel, Vladimír DRÁB and Ivo SEDLÁČEK. Ribotyping of Lactobacillus casei group strains isolated from dairy products. Online. Folia Microbiologica. Praha, 2005, vol. 50, No 3, p. 223-228. ISSN 0015-5632. [citováno 2024-04-24]
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Basic information
Original name Ribotyping of Lactobacillus casei group strains isolated from dairy products
Name in Czech Ribotypizace kmenů skupiny Lactobacillus casei izolovaných z mléčných výrobků
Authors ŠVEC, Pavel (203 Czech Republic, guarantor), Vladimír DRÁB (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic)
Edition Folia Microbiologica, Praha, 2005, 0015-5632.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 0.918
RIV identification code RIV/00216224:14310/05:00012712
Organization unit Faculty of Science
UT WoS 000232764300008
Keywords in English Lactobacillus casei group; ribotyping; identification; taxonomy
Tags identification, Lactobacillus casei group, ribotyping, taxonomy
Changed by Changed by: doc. RNDr. Pavel Švec, Ph.D., učo 1098. Changed: 18/6/2009 12:12.
Abstract
A series of lactobacilli isolated from dairy products were characterized using biotyping and ribotyping with EcoRI and HindIII restriction enzymes. Biotyping assigned 14 strains as Lactobacillus casei, 6 strains as Lactobacillus paracasei subsp. paracasei and 12 as Lactobacillus rhamnosus. The obtained ribotype patterns separated all analysed strains into two clearly distinguished groups corresponding to L. rhamnosus and L. casei/L. paracasei subsp. paracasei species. The HindIII ribotypes of individual strains representing these two groups were visually very similar. In contrast, EcoRI ribotyping revealed high intraspecies variability. All ribotypes of Lactobacillus casei and Lactobacillus paracasei subsp. paracasei dairy strains were very close and some strains even shared identical ribotype profiles. The type strains L. casei CCM 7088T (=ATCC 393T) and Lactobacillus zeae CCM 7069T revealing similar ribopatterns formed a separated subcluster using both restriction enzymes. In contrast, the ribotype profile of L. casei CCM 7089 (=ATCC 334) was very close to ribopatterns obtained from the dairy strains. These results support synonymy of L. casei and L. paracasei species revealed by other studies as well as reclassification of the type strain L. casei CCM 7088T as L. zeae and designation of L. casei CCM 7089 as the neotype strain.
Abstract (in Czech)
Ribotypizací s restrikčnímy enzymy EcoRI a HindIII byla charakterizována skupina laktobacilů izolovaná z mléčných produktů.
Links
GP204/02/D099, research and development projectName: Knihovna ribotypizačních profilů typových kultur bakterií
Investor: Czech Science Foundation, Database of the ribotype profiles of bacterial type strains
MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
QE1382, research and development projectName: Využití probiotik v mléčných výrobcích pro zvýšení jejich kvality, nutriční hodnoty a zdravotní bezpečnosti
Investor: Ministry of Agriculture of the CR, Utilization of probiotics in dairy products to improve their quality, nutrition value and health safety
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