Ribotyping of Lactobacillus casei group strains isolated from dairy products

ŠVEC, Pavel, Vladimír DRÁB and Ivo SEDLÁČEK. Ribotyping of Lactobacillus casei group strains isolated from dairy products. Folia Microbiologica. Praha, 2005, vol. 50, No 3, p. 223-228. ISSN 0015-5632.

Basic information

Original name

Ribotyping of Lactobacillus casei group strains isolated from dairy products

Name in Czech

Ribotypizace kmenů skupiny Lactobacillus casei izolovaných z mléčných výrobků

Authors

ŠVEC, Pavel (203 Czech Republic, guarantor), Vladimír DRÁB (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic)

Edition

Folia Microbiologica, Praha, 2005, 0015-5632

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.918

RIV identification code

RIV/00216224:14310/05:00012712

Organization unit

Faculty of Science

UT WoS

000232764300008

Keywords in English

Lactobacillus casei group; ribotyping; identification; taxonomy

Tags

identification, Lactobacillus casei group, ribotyping, taxonomy

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Abstract

V originále

A series of lactobacilli isolated from dairy products were characterized using biotyping and ribotyping with EcoRI and HindIII restriction enzymes. Biotyping assigned 14 strains as Lactobacillus casei, 6 strains as Lactobacillus paracasei subsp. paracasei and 12 as Lactobacillus rhamnosus. The obtained ribotype patterns separated all analysed strains into two clearly distinguished groups corresponding to L. rhamnosus and L. casei/L. paracasei subsp. paracasei species. The HindIII ribotypes of individual strains representing these two groups were visually very similar. In contrast, EcoRI ribotyping revealed high intraspecies variability. All ribotypes of Lactobacillus casei and Lactobacillus paracasei subsp. paracasei dairy strains were very close and some strains even shared identical ribotype profiles. The type strains L. casei CCM 7088T (=ATCC 393T) and Lactobacillus zeae CCM 7069T revealing similar ribopatterns formed a separated subcluster using both restriction enzymes. In contrast, the ribotype profile of L. casei CCM 7089 (=ATCC 334) was very close to ribopatterns obtained from the dairy strains. These results support synonymy of L. casei and L. paracasei species revealed by other studies as well as reclassification of the type strain L. casei CCM 7088T as L. zeae and designation of L. casei CCM 7089 as the neotype strain.

In Czech

Ribotypizací s restrikčnímy enzymy EcoRI a HindIII byla charakterizována skupina laktobacilů izolovaná z mléčných produktů.

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Links

GP204/02/D099, research and development project	Name: Knihovna ribotypizačních profilů typových kultur bakterií Investor: Czech Science Foundation, Database of the ribotype profiles of bacterial type strains
MSM0021622416, plan (intention)	Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
QE1382, research and development project	Name: Využití probiotik v mléčných výrobcích pro zvýšení jejich kvality, nutriční hodnoty a zdravotní bezpečnosti Investor: Ministry of Agriculture of the CR, Utilization of probiotics in dairy products to improve their quality, nutrition value and health safety