Effect of acute and repeated administration of methamphetamine
on the activity of CYP2D in isolated perfused rat liver

J 2005

Effect of acute and repeated administration of methamphetamine on the activity of CYP2D in isolated perfused rat liver

DOSTÁLEK, Miroslav, Jana PISTOVČÁKOVÁ, Veronika MINAŘÍKOVÁ, Jan JUŘICA, Josef TOMANDL et. al.

Basic information

Original name

Effect of acute and repeated administration of methamphetamine on the activity of CYP2D in isolated perfused rat liver

Name in Czech

Vliv akutního a opakovaného podávání metamfetaminu na aktivitu CYP2D na modelu izolovaných perfundovaných jater

Authors

DOSTÁLEK, Miroslav (203 Czech Republic), Jana PISTOVČÁKOVÁ (703 Slovakia), Veronika MINAŘÍKOVÁ (203 Czech Republic), Jan JUŘICA (203 Czech Republic), Josef TOMANDL (203 Czech Republic) and Eva HADAŠOVÁ (203 Czech Republic, guarantor)

Edition

Acta Veterinaria Brno, Brno, VFU Brno, 2005, 0001-7213

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.353

RIV identification code

RIV/00216224:14110/05:00014068

Organization unit

Faculty of Medicine

UT WoS

000232717000003

Keywords in English

Methamphetamine; Dextromethorphan; CYP2D; Liver; isolated perfused; Rat

Abstract

ORIG CZ

V originále

The aim of this study was to ascertain the influence of acute and repeated methamphetamine administration on metabolic activity of CYP2D2. The study was performed in the model of isolated perfused rat liver using dextromethorphan as a specific marker of CYP2D2 activity. Acute administration of methamphetamine (10, 20 or 40 mg/kg, i.p., once daily for two days) significantly increased dextrorphan O-formation in the isolated liver mediated by CYP2D2 at all investigated doses in the 120th min of liver perfusion. Repeated administration of methamphetamine (10, 20 or 40 mg/kg, i.p., once daily for six days) significantly stimulated dextromethorphan O-demethylation via CYP2D2 dose-dependently. Thus, an induction of CYP2D2 metabolic activity was proved in these experiments. With regard to a high level of homology between human and rat CYP isoform studied, the results may have clinical impact in pharmacotherapy of methamphetamine abusers.

In Czech

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Links

MSM 141100001, plan (intention)

Name: Plasticita řídících systémů centrálního nervového systému a možnosti jejího ovlivnění

Citovat

DOSTÁLEK, Miroslav, Jana PISTOVČÁKOVÁ, Veronika MINAŘÍKOVÁ, Jan JUŘICA, Josef TOMANDL and Eva HADAŠOVÁ. Effect of acute and repeated administration of methamphetamine on the activity of CYP2D in isolated perfused rat liver. Acta Veterinaria Brno. Brno: VFU Brno, 2005, vol. 74, No 3, p. 339-345. ISSN 0001-7213.

@article{584982,
   author = {Dostálek, Miroslav and Pistovčáková, Jana and Minaříková, Veronika and Juřica, Jan and Tomandl, Josef and Hadašová, Eva},
   article_location = {Brno},
   article_number = {3},
   keywords = {Methamphetamine; Dextromethorphan; CYP2D; Liver; isolated perfused; Rat},
   language = {eng},
   issn = {0001-7213},
   journal = {Acta Veterinaria Brno},
   title = {Effect of acute and repeated administration of methamphetamine on the activity of CYP2D in isolated perfused rat liver},
   volume = {74},
   year = {2005}
}

TY  - JOUR
ID  - 584982
AU  - Dostálek, Miroslav - Pistovčáková, Jana - Minaříková, Veronika - Juřica, Jan - Tomandl, Josef - Hadašová, Eva
PY  - 2005
TI  - Effect of acute and repeated administration of methamphetamine on the activity of CYP2D in isolated perfused rat liver
JF  - Acta Veterinaria Brno
VL  - 74
IS  - 3
SP  - 339-345
EP  - 339-345
PB  - VFU Brno
SN  - 00017213
KW  - Methamphetamine
KW  - Dextromethorphan
KW  - CYP2D
KW  - Liver
KW  - isolated perfused
KW  - Rat
N2  - The aim of this study was to ascertain the influence of acute and repeated methamphetamine administration on metabolic activity of CYP2D2. The study was performed in the model of isolated perfused rat liver using dextromethorphan as a specific marker of CYP2D2 activity. Acute administration of methamphetamine (10, 20 or 40 mg/kg, i.p., once daily for two days) significantly increased dextrorphan O-formation in the isolated liver mediated by CYP2D2 at all investigated doses in the 120th min of liver perfusion. Repeated administration of methamphetamine (10, 20 or 40 mg/kg, i.p., once daily for six days) significantly stimulated dextromethorphan O-demethylation via CYP2D2 dose-dependently. Thus, an induction of CYP2D2 metabolic activity was proved in these experiments. With regard to a high level of homology between human and rat CYP isoform studied, the results may have clinical impact in pharmacotherapy of methamphetamine abusers.
ER  -

DOSTÁLEK, Miroslav, Jana PISTOVČÁKOVÁ, Veronika MINAŘÍKOVÁ, Jan JUŘICA, Josef TOMANDL and Eva HADAŠOVÁ. Effect of acute and repeated administration of methamphetamine on the activity of CYP2D in isolated perfused rat liver. \textit{Acta Veterinaria Brno}. Brno: VFU Brno, 2005, vol.~74, No~3, p.~339-345. ISSN~0001-7213.

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