Indirect quantification of protein modification degree
HAVLIŠ, Jan, Ondrej ŠEDO and Zbyněk ZDRÁHAL. Indirect quantification of protein modification degree. In Book of abstracts 2nd Czech Proteomic Conference. 2005. |
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Basic information | |
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Original name | Indirect quantification of protein modification degree |
Name in Czech | Nepřímé stanovení rozsahu modifikace proteinu |
Authors | HAVLIŠ, Jan (203 Czech Republic, guarantor), Ondrej ŠEDO (203 Czech Republic) and Zbyněk ZDRÁHAL (203 Czech Republic). |
Edition | Book of abstracts 2nd Czech Proteomic Conference, 2005. |
Other information | |
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Original language | English |
Type of outcome | Conference abstract |
Field of Study | 10600 1.6 Biological sciences |
Country of publisher | Czech Republic |
Confidentiality degree | is not subject to a state or trade secret |
RIV identification code | RIV/00216224:14310/05:00014091 |
Organization unit | Faculty of Science |
Keywords in English | protein modification degree; post-translation modification; mass spectrometry |
Tags | mass spectrometry, post-translation modification, protein modification degree |
Changed by | Changed by: doc. Mgr. Jan Havliš, Dr., učo 743. Changed: 9/4/2010 14:57. |
Abstract |
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In our work, we present a general, indirect method, using which it is possible to quantify the percentage of the given amino acid modified in total in a protein sequence (protein modification degree, PMD). From the knowledge of the identity of amino acid residues modifiable during post-translation process, PMD and its changes during time of particular amino acid residue can be determined. Such a quantification can be achieved by means of easy stable isotope modification (via 18O-labelling by trypsination) and consequential MALDI TOF MS analysis. |
Abstract (in Czech) |
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Tato práce prezentuje obecnou, nepřímou metodu, pomocí níž je možné stanovit rozsah celkové modifikace dané aminokyseliny proteinu (protein modification degree, PMD). Známe-li identitu aminokyseliny modifikovatelné posttranslační modifikací, PMD a její změny ve vztahu k těmto aminokyselin mohou být takto stanoveny. Toto stanovení je dosaženo snadným značením stabilními izotopy (značení 18O v průběhu stěpení trypsinem) a následnou MALDI TOF MS analýzou. |
Links | |
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MSM0021622413, plan (intention) | Name: Proteiny v metabolismu a při interakci organismů s prostředím |
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment | |
MSM0021622415, plan (intention) | Name: Molekulární podstata buněčných a tkáňových regulací |
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations |
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