Indirect quantification of protein modification degree

HAVLIŠ, Jan, Ondrej ŠEDO and Zbyněk ZDRÁHAL. Indirect quantification of protein modification degree. In Book of abstracts 2nd Czech Proteomic Conference. 2005.

Basic information

• Original name: Indirect quantification of protein modification degree
• Name in Czech: Nepřímé stanovení rozsahu modifikace proteinu
• Authors: HAVLIŠ, Jan (203 Czech Republic, guarantor), Ondrej ŠEDO (203 Czech Republic) and Zbyněk ZDRÁHAL (203 Czech Republic).
• Edition: Book of abstracts 2nd Czech Proteomic Conference, 2005.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/05:00014091
• Organization unit: Faculty of Science
• Keywords in English: protein modification degree; post-translation modification; mass spectrometry
• Tags: mass spectrometry, post-translation modification, protein modification degree

Abstract

In our work, we present a general, indirect method, using which it is possible to quantify the percentage of the given amino acid modified in total in a protein sequence (protein modification degree, PMD). From the knowledge of the identity of amino acid residues modifiable during post-translation process, PMD and its changes during time of particular amino acid residue can be determined. Such a quantification can be achieved by means of easy stable isotope modification (via 18O-labelling by trypsination) and consequential MALDI TOF MS analysis.

Abstract (in Czech)

Tato práce prezentuje obecnou, nepřímou metodu, pomocí níž je možné stanovit rozsah celkové modifikace dané aminokyseliny proteinu (protein modification degree, PMD). Známe-li identitu aminokyseliny modifikovatelné posttranslační modifikací, PMD a její změny ve vztahu k těmto aminokyselin mohou být takto stanoveny. Toto stanovení je dosaženo snadným značením stabilními izotopy (značení 18O v průběhu stěpení trypsinem) a následnou MALDI TOF MS analýzou.

Links

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations