Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L

KOSTLÁNOVÁ, Nikola, Edward MITCHELL, Nechama GILBOA-GARBER, Michaela WIMMEROVÁ a Anne IMBERTY. Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L. In Materials and Structure in Chemistry, Biology, Physics and Technology. ARS Print, Praha: Krystalografická společnost, R. Kužel, 2005, s. 148-148.

Základní údaje

• Originální název: Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
• Název česky: Náhled do strukturně funkčních vztahů lektinů bakterie Ralstonia solanacearum RSL, RS-IIL, RS20L
• Název anglicky: Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
• Autoři: KOSTLÁNOVÁ, Nikola (203 Česká republika), Edward MITCHELL (826 Velká Británie a Severní Irsko), Nechama GILBOA-GARBER (376 Izrael), Michaela WIMMEROVÁ (203 Česká republika, garant) a Anne IMBERTY (250 Francie).
• Vydání: ARS Print, Praha, Materials and Structure in Chemistry, Biology, Physics and Technology, od s. 148-148, 1 s. 2005.
• Nakladatel: Krystalografická společnost, R. Kužel

Další údaje

• Originální jazyk: čeština
• Typ výsledku: Stať ve sborníku
• Obor: 10600 1.6 Biological sciences
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/05:00014094
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova anglicky: lectin; crystallography; Ralstonia solanacearum
• Štítky: CRYSTALLOGRAPHY, lectin, ralstonia solanacearum

Anotace

Lectins are a class of proteins of non-immune and non-enzymatic origin that bind carbohydrates specifically and reversibly. They express numerous biological activities, nearly all of which are based on their acting as recognition determinants in diverse biological processes including fertilization, pathogen-cell adhesion and recognition, inflammatory response and others. A number of pathogen microorganisms utilize lectin-carbohydrate interaction to recognized and infect host organism. The comprehension of the molecular mechanisms which gives a pathogenic bacterium the ability to invade, colonize and reorient the physiopathology of its host is a goal of primary importance and such studies may direct the conception of new strategies to fight these pathogenic agents1. Ralstonia solanacearum is soil-borne bacterium, which belongs to the group of beta-proteobacteria. It is responsible for bacterial wilts on more than 200 plant species including potato, tomato banana and others economically important corps 1. R. solanacearum, which is capable of living for prolonged periods in the soil, infects its hosts beginning with the root system and presents a very strong tropism for the xylem vessels. Its extensive multiplication in the water-conducting system leads to a systemic infection of the plant. This contribution describes three lectins RSL (9.9 kDa) 2, RS-IIL (11.6 kDa) 3 and RS20L (20 kDa) that have been found in R. solanacearum extract and purified using affinity chromatography. All lectins were crystallized by vapor diffusion and high and ultra-high (in case of 0.94Å resolution of RSL/-methyl fucose) resolution data were collected at ESRF, Grenoble, France. The structural data have been supplemented by ITC microcalorimetry, surface plasmon resonance studies and ELLA tests defining lectins specificity to carbohydrates including those, which are commonly present in nature and may be the target for the lectins in soil.

Anotace anglicky

Lectins are a class of proteins of non-immune and non-enzymatic origin that bind carbohydrates specifically and reversibly. They express numerous biological activities, nearly all of which are based on their acting as recognition determinants in diverse biological processes including fertilization, pathogen-cell adhesion and recognition, inflammatory response and others. A number of pathogen microorganisms utilize lectin-carbohydrate interaction to recognized and infect host organism. The comprehension of the molecular mechanisms which gives a pathogenic bacterium the ability to invade, colonize and reorient the physiopathology of its host is a goal of primary importance and such studies may direct the conception of new strategies to fight these pathogenic agents1. Ralstonia solanacearum is soil-borne bacterium, which belongs to the group of beta-proteobacteria. It is responsible for bacterial wilts on more than 200 plant species including potato, tomato banana and others economically important corps 1. R. solanacearum, which is capable of living for prolonged periods in the soil, infects its hosts beginning with the root system and presents a very strong tropism for the xylem vessels. Its extensive multiplication in the water-conducting system leads to a systemic infection of the plant. This contribution describes three lectins RSL (9.9 kDa) 2, RS-IIL (11.6 kDa) 3 and RS20L (20 kDa) that have been found in R. solanacearum extract and purified using affinity chromatography. All lectins were crystallized by vapor diffusion and high and ultra-high (in case of 0.94Å resolution of RSL/-methyl fucose) resolution data were collected at ESRF, Grenoble, France. The structural data have been supplemented by ITC microcalorimetry, surface plasmon resonance studies and ELLA tests defining lectins specificity to carbohydrates including those, which are commonly present in nature and may be the target for the lectins in soil.

Návaznosti

• MSM0021622413, záměr: Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím