Detailed Information on Publication Record
2005
Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
KOSTLÁNOVÁ, Nikola, Edward MITCHELL, Nechama GILBOA-GARBER, Michaela WIMMEROVÁ, Anne IMBERTY et. al.Basic information
Original name
Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
Name in Czech
Náhled do strukturně funkčních vztahů lektinů bakterie Ralstonia solanacearum RSL, RS-IIL, RS20L
Name (in English)
Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
Authors
KOSTLÁNOVÁ, Nikola (203 Czech Republic), Edward MITCHELL (826 United Kingdom of Great Britain and Northern Ireland), Nechama GILBOA-GARBER (376 Israel), Michaela WIMMEROVÁ (203 Czech Republic, guarantor) and Anne IMBERTY (250 France)
Edition
ARS Print, Praha, Materials and Structure in Chemistry, Biology, Physics and Technology, p. 148-148, 1 pp. 2005
Publisher
Krystalografická společnost, R. Kužel
Other information
Language
Czech
Type of outcome
Stať ve sborníku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/05:00014094
Organization unit
Faculty of Science
Keywords in English
lectin; crystallography; Ralstonia solanacearum
Změněno: 3/11/2005 10:02, Mgr. Nikola Kostlánová, Ph.D.
V originále
Lectins are a class of proteins of non-immune and non-enzymatic origin that bind carbohydrates specifically and reversibly. They express numerous biological activities, nearly all of which are based on their acting as recognition determinants in diverse biological processes including fertilization, pathogen-cell adhesion and recognition, inflammatory response and others. A number of pathogen microorganisms utilize lectin-carbohydrate interaction to recognized and infect host organism. The comprehension of the molecular mechanisms which gives a pathogenic bacterium the ability to invade, colonize and reorient the physiopathology of its host is a goal of primary importance and such studies may direct the conception of new strategies to fight these pathogenic agents1. Ralstonia solanacearum is soil-borne bacterium, which belongs to the group of beta-proteobacteria. It is responsible for bacterial wilts on more than 200 plant species including potato, tomato banana and others economically important corps 1. R. solanacearum, which is capable of living for prolonged periods in the soil, infects its hosts beginning with the root system and presents a very strong tropism for the xylem vessels. Its extensive multiplication in the water-conducting system leads to a systemic infection of the plant. This contribution describes three lectins RSL (9.9 kDa) 2, RS-IIL (11.6 kDa) 3 and RS20L (20 kDa) that have been found in R. solanacearum extract and purified using affinity chromatography. All lectins were crystallized by vapor diffusion and high and ultra-high (in case of 0.94Å resolution of RSL/-methyl fucose) resolution data were collected at ESRF, Grenoble, France. The structural data have been supplemented by ITC microcalorimetry, surface plasmon resonance studies and ELLA tests defining lectins specificity to carbohydrates including those, which are commonly present in nature and may be the target for the lectins in soil.
In English
Lectins are a class of proteins of non-immune and non-enzymatic origin that bind carbohydrates specifically and reversibly. They express numerous biological activities, nearly all of which are based on their acting as recognition determinants in diverse biological processes including fertilization, pathogen-cell adhesion and recognition, inflammatory response and others. A number of pathogen microorganisms utilize lectin-carbohydrate interaction to recognized and infect host organism. The comprehension of the molecular mechanisms which gives a pathogenic bacterium the ability to invade, colonize and reorient the physiopathology of its host is a goal of primary importance and such studies may direct the conception of new strategies to fight these pathogenic agents1. Ralstonia solanacearum is soil-borne bacterium, which belongs to the group of beta-proteobacteria. It is responsible for bacterial wilts on more than 200 plant species including potato, tomato banana and others economically important corps 1. R. solanacearum, which is capable of living for prolonged periods in the soil, infects its hosts beginning with the root system and presents a very strong tropism for the xylem vessels. Its extensive multiplication in the water-conducting system leads to a systemic infection of the plant. This contribution describes three lectins RSL (9.9 kDa) 2, RS-IIL (11.6 kDa) 3 and RS20L (20 kDa) that have been found in R. solanacearum extract and purified using affinity chromatography. All lectins were crystallized by vapor diffusion and high and ultra-high (in case of 0.94Å resolution of RSL/-methyl fucose) resolution data were collected at ESRF, Grenoble, France. The structural data have been supplemented by ITC microcalorimetry, surface plasmon resonance studies and ELLA tests defining lectins specificity to carbohydrates including those, which are commonly present in nature and may be the target for the lectins in soil.
Links
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