Detailed Information on Publication Record
2005
13C-Detected Experiments for NMR Spectroscopy of Nucleic Acids
FIALA, Radovan and Vladimír SKLENÁŘBasic information
Original name
13C-Detected Experiments for NMR Spectroscopy of Nucleic Acids
Name in Czech
Experimenty s přímou uhlíkovou detekcí pro NMR spektroskopii nukleových kyselin
Authors
FIALA, Radovan and Vladimír SKLENÁŘ
Edition
1. vyd. Veldhoven, EUROMAR/EENC 2005, p. 388-388, 1 pp. 2005
Publisher
Utrecht University
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Faculty of Science
Keywords in English
NMR spectroscopy; direct carbon detection; nucleic acid
Změněno: 3/11/2005 17:59, doc. RNDr. Radovan Fiala, CSc.
V originále
Nucleic acid bases contain a number of carbon atoms without directly attached protons. These include carbonyl groups in cytosine, thymine, uracil, and guanine, the C4 atom in cytosine, C5 in thymine, C4, C5, C6 in adenine, and C2, C4, and C5 in guanine. The direct detection of these carbon atoms offers an alternative method for obtaining their chemical shift values and/or data for investigation of the dynamics of nucleic acid bases. Furthermore, the chemical shifts of the amino nitrogen atoms whose protons are broadened beyond detection due to the exchange with water can be obtained using a 13C-detected 13C-15N correlation experiment. In the sugar moiety, the carbon detected experiments are suitable for obtaining 1H-13C correlations in the cases where the proton signal is obscured by the large water peak.
In Czech
Metody využívající přímé detekce jader uhlíku C-13 v NMR spektroskopii nukleových kyselin
Links
| MSM0021622413, plan (intention) |
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