Activation of different defence-related genes expression by
ergosterol

D 2005

Activation of different defence-related genes expression by ergosterol

LOCHMAN, Jan and Vladimír MIKEŠ

Basic information

Original name

Activation of different defence-related genes expression by ergosterol

Name in Czech

Aktivace exprese obranných genů ergosterolem

Authors

LOCHMAN, Jan (203 Czech Republic) and Vladimír MIKEŠ (203 Czech Republic, guarantor)

Edition

Budapest, FEBS Journal, Abstract of 30th FEBS Congress, p. 470-470, 2005

Publisher

Blackwell publishing

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

40106 Agronomy, plant breeding and plant protection;

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/05:00014115

Organization unit

Faculty of Science

ISBN

nebylo pridelen

UT WoS

000234826102422

Keywords in English

Elicitor; ergosterol; plant defense

Abstract

ORIG CZ

V originále

Ergosterol is the main sterol of most fungi. Production of reactive oxygen species by NADPH oxidase, alkalinization of extracellular space and increase of cytosolic concentration of calcium after the ergosterol treatment on tobacco cells was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells, even in nano-molar concentrations. Ergosterol activates a signal pathway including phospholipase A2 and protein kinase C. Here, we report about the expression of some defence-related genes after the treatment of tobacco cells and plants with nano-molar concentration of ergosterol. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors together with enzymes as phenylalanine ammonia lyase, sesquiterpene cyclase and lipooxygenase was observed by a real-time PCR,. In addition, the concentrations of some phenolic compounds and phytoalexins increased in time. On the other hand, ergosterol did not provoke any leaf necrosis or cell death and the accumulation of PR proteins was slower when compared with another well defined elicitor cryptogein from Phytophthora cryptogea. These data shows that ergosterol is able to activate expression of a number of defence genes and could increase the resistance of plants against the pathogens.

In Czech

Aktivace exprese obranných genů ergosterolem

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

LOCHMAN, Jan and Vladimír MIKEŠ. Activation of different defence-related genes expression by ergosterol. In FEBS Journal, Abstract of 30th FEBS Congress. Budapest: Blackwell publishing, 2005, p. 470. ISBN nebylo pridelen.

@inproceedings{586702,
   author = {Lochman, Jan and Mikeš, Vladimír},
   address = {Budapest},
   booktitle = {FEBS Journal, Abstract of 30th FEBS Congress},
   keywords = {Elicitor; ergosterol; plant defense},
   language = {eng},
   location = {Budapest},
   isbn = {nebylo pridelen},
   pages = {470-470},
   publisher = {Blackwell publishing},
   title = {Activation of different defence-related genes expression by ergosterol},
   year = {2005}
}

TY  - JOUR
ID  - 586702
AU  - Lochman, Jan - Mikeš, Vladimír
PY  - 2005
TI  - Activation of different defence-related genes expression by ergosterol
PB  - Blackwell publishing
CY  - Budapest
SN  - nebylo pridelen
KW  - Elicitor
KW  - ergosterol
KW  - plant defense
N2  - Ergosterol is the main sterol of most fungi. Production of reactive oxygen species by NADPH oxidase, alkalinization of extracellular space and increase of cytosolic concentration of calcium after the ergosterol treatment on tobacco cells was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells, even in nano-molar concentrations. Ergosterol activates a signal pathway including phospholipase A2 and protein kinase C. Here, we report about the expression of some defence-related genes after the treatment of tobacco cells and plants with nano-molar concentration of ergosterol. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors together with enzymes as phenylalanine ammonia lyase, sesquiterpene cyclase and lipooxygenase was observed by a real-time PCR,. In addition, the concentrations of some phenolic compounds and phytoalexins increased in time. On the other hand, ergosterol did not provoke any leaf necrosis or cell death and the accumulation of PR proteins was slower when compared with another well defined elicitor cryptogein from Phytophthora cryptogea. These data shows that ergosterol is able to activate expression of a number of defence genes and could increase the resistance of plants against the pathogens.
ER  -

LOCHMAN, Jan and Vladimír MIKEŠ. Activation of different defence-related genes expression by ergosterol. In \textit{FEBS Journal, Abstract of 30th FEBS Congress}. Budapest: Blackwell publishing, 2005, p.~470. ISBN~nebylo~pridelen.

Detailed Information on Publication Record