D 2005

New insight into mediator function of yeast Rad52 protein

KREJCI, Lumir, Iben PLATE, Patrick SUNG, Michael SEHORN, Wendy BUSSEN et. al.

Basic information

Original name

New insight into mediator function of yeast Rad52 protein

Name in Czech

Nový pohled na mediátorovou funkci proteinu Rad52

Authors

KREJCI, Lumir, Iben PLATE, Patrick SUNG, Michael SEHORN, Wendy BUSSEN and Uffe H. MORTENSEN

Edition

Bratislava, Slovakia, XXIIth International Conference on Yeast Genetics and Molecular Biology, 90 pp. 2005

Publisher

ASM Conferences Program

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Organization unit

Faculty of Science

Keywords in English

Recombination; DNA repair; Rad52; mediator; yeast
Změněno: 30/6/2009 22:56, doc. Mgr. Lumír Krejčí, Ph.D.

Abstract

V originále

Homologous recombination (HR) represents an important means for the repair of chromosome breaks induced by exogenous agents (e.g. ionizing radiation) or that arise endogenously (e.g. replication of a damaged DNA template). The elimination of these breaks is critical for the maintenance of genome stability. DNA strand break repair by HR is mediated by genes of the RAD52 epistasis group, whose structure and function are highly conserved among eukaryotes. Rad52 protein plays central role in this process. It binds and anneals complemtary ssDNA, self-associate to form multimeric rings, interacts with Rad51, and promotes nucleation of Rad51 onto ssDNA. Here, we show that Rad52 contains second DNA binding domain that is capable of mediating Rad51-catalyzed strand exchange reaction. The molecular mechanism of Rad52 function in homologous recombination is disccused.

In Czech

V této praci prezentujeme, že Rad52 protein obsahuje druhou DNA vazebnou doménu, která je schopna zprostredkovat Rad51-katalyzovanou výměnu řetězců. Diskutujeme molekulární mechanismus homologní rekombinace.

Links

MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment