DNA Binding and Strand Annealing Properties of the Human BRCA2 DNA Binding Domain

KREJCI, Lumir, Julia ETCHIN, San Joe FILIPPO and Patrick SUNG. DNA Binding and Strand Annealing Properties of the Human BRCA2 DNA Binding Domain (DNA Binding and Strand Annealing Properties of the Human BRCA2 DNA binding Domain). In Era of Hope 2005 - Department of Defense Breast Cancer Research Program Meeting. Philadelphia, USA: U.S. Army Medical Research and Materiel Command, 2005, 89 pp.

Basic information

Original name

DNA Binding and Strand Annealing Properties of the Human BRCA2 DNA Binding Domain

Name in Czech

DNA vazebné a hybridizacní vlastnosti DNA vazebne doménz lidského BRCA2 proteinu

Authors

KREJCI, Lumir, Julia ETCHIN, San Joe FILIPPO and Patrick SUNG

Edition

Philadelphia, USA, Era of Hope 2005 - Department of Defense Breast Cancer Research Program Meeting, 89 pp. 2005

Publisher

U.S. Army Medical Research and Materiel Command

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Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Science

Keywords in English

DNA repair; recombination; breast cancer; BRCA2; DNA binding

Tags

BRCA2, breast cancer, DNA binding, DNA repair, recombination

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Abstract

V originále

Mutations in the BRCA2 (breast cancer susceptibility gene 2) gene give rise to a significant portion of familial breast cancers. BRCA2 associates with Rad51, a key member of the RAD52 group, through a series of conserved modules called BRC repeats. Rad51 has a recombinase activity that mediates the formation of DNA joints between the injured chromosome and an intact homologue during HR. In mediating HR reactions, Rad51 nucleates onto single-stranded (ss) DNA derived from lesion processing to form a highly ordered nucleoprotein filament structure, often referred to as the presynaptic filament. After locating a DNA homologue, the presynaptic filament catalyzes pairing with the homologue to form a nascent DNA joint called a D-loop, the length of which is extended by DNA strand exchange. Concurrently, DNA synthesis commences to replace the genetic information lost during the lesion processing step. Subsequent steps include resolution of recombination intermediates and ligation to complete the recombination/repair reaction.

In Czech

Mutations in the BRCA2 (breast cancer susceptibility gene 2) gene give rise to a significant portion of familial breast cancers. BRCA2 associates with Rad51, a key member of the RAD52 group, through a series of conserved modules called BRC repeats. Rad51 has a recombinase activity that mediates the formation of DNA joints between the injured chromosome and an intact homologue during HR. In mediating HR reactions, Rad51 nucleates onto single-stranded (ss) DNA derived from lesion processing to form a highly ordered nucleoprotein filament structure, often referred to as the presynaptic filament. After locating a DNA homologue, the presynaptic filament catalyzes pairing with the homologue to form a nascent DNA joint called a D-loop, the length of which is extended by DNA strand exchange. Concurrently, DNA synthesis commences to replace the genetic information lost during the lesion processing step. Subsequent steps include resolution of recombination intermediates and ligation to complete the recombination/repair reaction.

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Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment