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@inproceedings{590965,
author = {Nováková, Marie and Provazník, Ivo and Bardoňová, Jana and Blaha, Milan and Táborská, Eva and Bochořáková, Hana and Paulová, Hana and Horký, Drahomír and Krejčířová, Lenka},
address = {Praha},
booktitle = {IFMBE Proceedings, EMBEC 05},
edition = {11},
keywords = {voltage sensitive dye; guinea pig myocardium; ECG; coronary flow; HPLC; electron microscopy},
language = {eng},
location = {Praha},
pages = {1-5},
publisher = {IFMBE},
title = {Effects of Voltage Sensitive Dye di-4-ANEPPS on Guinea Pig Myocardium},
year = {2005}
}
TY - JOUR
ID - 590965
AU - Nováková, Marie - Provazník, Ivo - Bardoňová, Jana - Blaha, Milan - Táborská, Eva - Bochořáková, Hana - Paulová, Hana - Horký, Drahomír - Krejčířová, Lenka
PY - 2005
TI - Effects of Voltage Sensitive Dye di-4-ANEPPS on Guinea Pig Myocardium
PB - IFMBE
CY - Praha
KW - voltage sensitive dye
KW - guinea pig myocardium
KW - ECG
KW - coronary flow
KW - HPLC
KW - electron microscopy
N2 - Voltage-sensitive dyes are used for recording monophasic action potentials (MAPs) in various heart preparations. Aim of this study was to prove that dye loading has no pharmacological or toxic effects to the preparation, e.g. to prove the viability and quality of the heart at the beginning of MAP recording. The hearts of fifteen guinea pigs were perfused according to Langendorf, loaded with the dye (di-4-ANEPPS) and washed out. During the experiment electrogram and mean coronary flow was recorded. High performance liquid chromatography (HPLC) was used to test on possible ischemic changes of the heart during loading and electron microscopy to check the microscopic changes in the dye exposed tissue. We observed direct effect of di-4-ANEPPS on conductive system and working myocardium of guinea pig heart (changes in heart rate and shape of electrogram). The mean coronary flow does not significantly change during the period of loading with the dye, however during washout decreases. Since no increased production of oxygen free radicals was found and no ultrastructural changes observed, we conclude that the dye application causes vasoconstriction in coronary system without functional or morphological consequences and that loading procedure does not damage the heart muscle and its viability.
ER -
NOVÁKOVÁ, Marie, Ivo PROVAZNÍK, Jana BARDOŇOVÁ, Milan BLAHA, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ, Hana PAULOVÁ, Drahomír HORKÝ and Lenka KREJČÍŘOVÁ. Effects of Voltage Sensitive Dye di-4-ANEPPS on Guinea Pig Myocardium. In \textit{IFMBE Proceedings, EMBEC 05}. 11th ed. Praha: IFMBE, 2005, p.~1-5.
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