PRODĚLALOVÁ, Jana, Alena ŠPANOVÁ and Bohuslav RITTICH. Application of PCR, rep-PCR and RAPD techniques for typing of Lactococcus lactis strains. Folia Microbiologica. Praha: Institute of Microbiology AS CR, 2005, vol. 50, No 2, p. 150-154. ISSN 0015-5632.
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Basic information
Original name Application of PCR, rep-PCR and RAPD techniques for typing of Lactococcus lactis strains
Name in Czech Využití technik PCR, rep-PCR a RAPD pro typizaci kmenů Lactococcus lactis
Authors PRODĚLALOVÁ, Jana (203 Czech Republic), Alena ŠPANOVÁ (203 Czech Republic) and Bohuslav RITTICH (203 Czech Republic, guarantor).
Edition Folia Microbiologica, Praha, Institute of Microbiology AS CR, 2005, 0015-5632.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.918
RIV identification code RIV/00216224:14310/05:00014203
Organization unit Faculty of Science
UT WoS 000230832900012
Keywords in English Lactococcus lactis; PCR; rep-PCR; RAPD
Tags Lactococcus lactis, PCR, RAPD, rep-PCR
Tags Reviewed
Changed by Changed by: doc. Ing. Bohuslav Rittich, CSc., učo 1639. Changed: 23/11/2006 08:47.
Abstract
A collection of 34 lactococcal strains were characterized using PCR for acmA gene, and for 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindromes-PCR (rep-PCR). PCR experiments enabled the genotypic identification of L. lactis strains by RAPD. Rep-PCR did not distinguish between L. lactis subspecies. In some cases, phenotypic classification of L. lactis subspecies did not correlate with genotypic characterizations.
Abstract (in Czech)
Soubor 34 kmenů laktokoků byl charakterizován pomocí PCR pro acmA a 16S rDNA geny,RAPD a rep-PCR. Genotypová identifikace kmenů L. lactisbyla umožněna použitím RAPD. Rep-PCR neumožnila rozlišení podruhů L. lactis. V některých případech fenotypická klasifikace podruhů L. lactis nekorelovala s genotypovou charakterizací.
Links
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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