Detailed Information on Publication Record
2006
High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser
MOSKOVETS, Eugene, Jan PREISLER, H. S. CHEN, Tomáš REJTAR, Viktor ANDREEV et. al.Basic information
Original name
High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser
Name in Czech
Vysoce výkonná axiální MALDI-TOF MS s 2 kHz laserem
Authors
MOSKOVETS, Eugene (643 Russian Federation), Jan PREISLER (203 Czech Republic, guarantor), H. S. CHEN (768 Togo), Tomáš REJTAR (203 Czech Republic), Viktor ANDREEV (643 Russian Federation) and Barry L. KARGER (840 United States of America)
Edition
Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2006, 0003-2700
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 5.646
RIV identification code
RIV/00216224:14310/06:00015575
Organization unit
Faculty of Science
UT WoS
000235195200050
Keywords in English
MALDI; TOF; mass spectrometry; time of flight; high-throughput
Změněno: 27/6/2009 23:56, prof. Mgr. Jan Preisler, Ph.D.
V originále
A high-throughput axial MALDI-TOF mass spectrometer utilizing a laser with a 2 kHz pulse repetition-rate was constructed and tested. This fast mass spectrometer provided a data acquisition rate 10 times faster than a commercially available (200 Hz) axial mass spectrometer, while maintaining comparable limits of detection (200 amole of Glu fib peptide). Mass resolution, only slightly less than the commercial instrument (10,000 vs. 14,000), was sufficient for baseline resolution of isotopic clusters of peptides with m/z <2700. A new method of mass calibration, which combined a limited number of internal and external standards, provided the same 15 ppm mass accuracy over the entire sample plate on either instrument. Implementing the 2 kHz laser required a faster data acquisition system and high-voltage pulse electronics, together with a novel strategy for rapid sample plate movements during acquisition, to achieve a sample analysis rate of up to 2 spots per second (with 800 shots/spot). The overall performance of the fast MALDI-TOF MS instrument was demonstrated by the acquisition, in 12 minutes, of an LC-MS dataset from a plate of 650 fractions collected during LC separation of an 16O/18O differentially-labeled proteomic sample of a tryptic digest of an E.coli lysate.
In Czech
Byl zkonstruován a vyzkoušen vysoce výkonný axiální MALDI-TOF MS s 2 kHz laserem. Detekční limit cca 200 amole of Glu fib peptidu, hmotnostní rozlišení 10000, správnost stanovení m/z 15 ppm, rychlost analýzy až 2 vzorky za sekundu při 800 pulsech na vzorek. Demonstrace analýzy 650 frakcí z LC-MS během 12 minut. Další detaily v anglické anotaci.
Links
| GA203/03/0515, research and development project |
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