Amperometric flow-injection determination of phenolic compounds
using a biosensor with immobilised laccase, peroxidase and
tyrosinase

J 2005

Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase

SOLNÁ, Renáta and Petr SKLÁDAL

Basic information

Original name

Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase

Name in Czech

Amperometrický průtočný biosensor pro stanovení fenolických sloučenin s imobilisovanou lakasou, peroxidasou a tyrosinasou

Authors

SOLNÁ, Renáta (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor)

Edition

Electroanalysis, Weinheim, Wiley-VCH Verlag GmbH, 2005, 1040-0397

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.189

RIV identification code

RIV/00216224:14310/05:00014346

Organization unit

Faculty of Science

UT WoS

000234031500005

Keywords in English

electrochemical biosensor; enzyme electrode; phenol; catechol; waste water

Abstract

ORIG CZ

V originále

A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min. The preliminary studies showed that the compatibility of the above mentioned enzyme array enabled the multielectrode biosensor to be applied to real samples including industrial wastewaters and surface waters.

In Czech

Byl vyvinut multikanálový elektrochemický biosensor pro stanovení fenolických látek v odpadních vodách pomocí enzymů specifických k fenolům: lakasa, peroxidasy a tyrosinasa.

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

SOLNÁ, Renáta and Petr SKLÁDAL. Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase. Electroanalysis. Weinheim: Wiley-VCH Verlag GmbH, 2005, vol. 17, No 23, p. 2137-2146. ISSN 1040-0397.

@article{598204,
   author = {Solná, Renáta and Skládal, Petr},
   article_location = {Weinheim},
   article_number = {23},
   keywords = {electrochemical biosensor; enzyme electrode; phenol; catechol; waste water},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   title = {Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase},
   volume = {17},
   year = {2005}
}

TY  - JOUR
ID  - 598204
AU  - Solná, Renáta - Skládal, Petr
PY  - 2005
TI  - Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase
JF  - Electroanalysis
VL  - 17
IS  - 23
SP  - 2137-2146
EP  - 2137-2146
PB  - Wiley-VCH Verlag GmbH
SN  - 10400397
KW  - electrochemical biosensor
KW  - enzyme electrode
KW  - phenol
KW  - catechol
KW  - waste water
N2  - A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min. The preliminary studies showed that the compatibility of the above mentioned enzyme array enabled the multielectrode biosensor to be applied to real samples including industrial wastewaters and surface waters.
ER  -

SOLNÁ, Renáta and Petr SKLÁDAL. Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase. \textit{Electroanalysis}. Weinheim: Wiley-VCH Verlag GmbH, 2005, vol.~17, No~23, p.~2137-2146. ISSN~1040-0397.

Detailed Information on Publication Record