SOLNÁ, Renáta and Petr SKLÁDAL. Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase. Electroanalysis. Weinheim: Wiley-VCH Verlag GmbH, 2005, vol. 17, No 23, p. 2137-2146. ISSN 1040-0397.
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Basic information
Original name Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase
Name in Czech Amperometrický průtočný biosensor pro stanovení fenolických sloučenin s imobilisovanou lakasou, peroxidasou a tyrosinasou
Authors SOLNÁ, Renáta (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor).
Edition Electroanalysis, Weinheim, Wiley-VCH Verlag GmbH, 2005, 1040-0397.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.189
RIV identification code RIV/00216224:14310/05:00014346
Organization unit Faculty of Science
UT WoS 000234031500005
Keywords in English electrochemical biosensor; enzyme electrode; phenol; catechol; waste water
Tags catechol, electrochemical biosensor, Enzyme electrode, PHENOL, waste water
Changed by Changed by: prof. RNDr. Petr Skládal, CSc., učo 2202. Changed: 2/1/2006 13:27.
Abstract
A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min. The preliminary studies showed that the compatibility of the above mentioned enzyme array enabled the multielectrode biosensor to be applied to real samples including industrial wastewaters and surface waters.
Abstract (in Czech)
Byl vyvinut multikanálový elektrochemický biosensor pro stanovení fenolických látek v odpadních vodách pomocí enzymů specifických k fenolům: lakasa, peroxidasy a tyrosinasa.
Links
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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