DUBOVÝ, Petr, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ivana SVÍŽENSKÁ and Kateřina PEJCHALOVÁ. Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain (Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain.). In Folia Medica Cassoviensia. Košice: Institute of Neurobiology, Slovak Academy of Science. p. 22-22. ISBN 80-7097-607-1. 2005.
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Basic information
Original name Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain
Name in Czech Intra- a extraneuronální změny v imunofluorescenční lokalizaci prozánětlivých a protizánětlivých molekul v experimentálních modelech neuropatické bolesti
Authors DUBOVÝ, Petr (203 Czech Republic, guarantor), Radim JANČÁLEK (203 Czech Republic), Ilona KLUSÁKOVÁ (203 Czech Republic), Ivana SVÍŽENSKÁ (203 Czech Republic) and Kateřina PEJCHALOVÁ (203 Czech Republic).
Edition Košice, Folia Medica Cassoviensia, p. 22-22, 1 pp. 2005.
Publisher Institute of Neurobiology, Slovak Academy of Science
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 30000 3. Medical and Health Sciences
Country of publisher Slovakia
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/05:00012984
Organization unit Faculty of Medicine
ISBN 80-7097-607-1
Keywords in English dorsal root ganglia; TNF-alpha; TNFR1
Tags Dorsal root ganglia, TNF-alpha, TNFR1
Changed by Changed by: prof. RNDr. Petr Dubový, CSc., učo 698. Changed: 26/6/2009 10:39.
Abstract
Peripheral neuropathic pain has been investigated using rat peripheral nerve injury models including unilateral sciatic nerve transection or ligation, spinal nerve ligation or ventral root transection. The experimental models were utilized for the study of immunofluorescence (IF) staining for IL-1b, IL-6, TNFa and TNFaR1 in the rat DRG. Quantitative and qualitative IF changes in the DRG affected by unilateral nerve injury were compared with the contralateral ones removed from operated animals as well as naive rats. Ipsi- and contralateral DRG displayed increased IF for TNFa and TNFaR1 when compared with DRG of naive rats. The TNFa IF was increased in both DRG neurons and their satellite glial cells, while enhanced IF for TNFaR1 was observed only at the surface of satellite glial cells after various types of nerve injury. In addition, ipsilateral DRG exhibited an elevation of TNFa immunoreactivity by invaded ED-1+ macrophages. The IL-1b IF was increased in the DRG neurons following sciatic nerve constriction, but not after the nerve trasection. A higher level of IF for IL-6 was observed in the DRG neurons and their satellite glial cells following sciatic nerve ligature as well as ventral root transection.
Abstract (in Czech)
Intra- a extraneuronální změny v imunofluorescenční lokalizaci prozánětlivých a protizánětlivých molekul v experimentálních modelech neuropatické bolesti
Links
GA309/03/1199, research and development projectName: Změny v satelitních gliových buňkách a jejich extracelulární matrix po axotomii - podmínky pro tvorbu sympatických kolaterál ve spinálních gangliích ?
Investor: Czech Science Foundation
MSM0021622404, plan (intention)Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS
Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.
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