Intra- and extraneuronal changes of immunofluorescence staining
for inflammation-related molecules in the experimental models
of peripheral neuropathic pain

D 2005

Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain

DUBOVÝ, Petr, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ivana SVÍŽENSKÁ, Kateřina PEJCHALOVÁ et. al.

Basic information

Original name

Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain

Name in Czech

Intra- a extraneuronální změny v imunofluorescenční lokalizaci prozánětlivých a protizánětlivých molekul v experimentálních modelech neuropatické bolesti

Authors

DUBOVÝ, Petr (203 Czech Republic, guarantor), Radim JANČÁLEK (203 Czech Republic), Ilona KLUSÁKOVÁ (203 Czech Republic), Ivana SVÍŽENSKÁ (203 Czech Republic) and Kateřina PEJCHALOVÁ (203 Czech Republic)

Edition

Košice, Folia Medica Cassoviensia, p. 22-22, 1 pp. 2005

Publisher

Institute of Neurobiology, Slovak Academy of Science

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/05:00012984

Organization unit

Faculty of Medicine

ISBN

80-7097-607-1

Keywords in English

dorsal root ganglia; TNF-alpha; TNFR1

Abstract

ORIG CZ

V originále

Peripheral neuropathic pain has been investigated using rat peripheral nerve injury models including unilateral sciatic nerve transection or ligation, spinal nerve ligation or ventral root transection. The experimental models were utilized for the study of immunofluorescence (IF) staining for IL-1b, IL-6, TNFa and TNFaR1 in the rat DRG. Quantitative and qualitative IF changes in the DRG affected by unilateral nerve injury were compared with the contralateral ones removed from operated animals as well as naive rats. Ipsi- and contralateral DRG displayed increased IF for TNFa and TNFaR1 when compared with DRG of naive rats. The TNFa IF was increased in both DRG neurons and their satellite glial cells, while enhanced IF for TNFaR1 was observed only at the surface of satellite glial cells after various types of nerve injury. In addition, ipsilateral DRG exhibited an elevation of TNFa immunoreactivity by invaded ED-1+ macrophages. The IL-1b IF was increased in the DRG neurons following sciatic nerve constriction, but not after the nerve trasection. A higher level of IF for IL-6 was observed in the DRG neurons and their satellite glial cells following sciatic nerve ligature as well as ventral root transection.

In Czech

Intra- a extraneuronální změny v imunofluorescenční lokalizaci prozánětlivých a protizánětlivých molekul v experimentálních modelech neuropatické bolesti

Links

GA309/03/1199, research and development project

Name: Změny v satelitních gliových buňkách a jejich extracelulární matrix po axotomii - podmínky pro tvorbu sympatických kolaterál ve spinálních gangliích ?

Investor: Czech Science Foundation

MSM0021622404, plan (intention)

Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.

Citovat

DUBOVÝ, Petr, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ivana SVÍŽENSKÁ and Kateřina PEJCHALOVÁ. Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain (Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain.). In Folia Medica Cassoviensia. Košice: Institute of Neurobiology, Slovak Academy of Science, 2005, p. 22-22. ISBN 80-7097-607-1.

@inproceedings{602084,
   author = {Dubový, Petr and Jančálek, Radim and Klusáková, Ilona and Svíženská, Ivana and Pejchalová, Kateřina},
   address = {Košice},
   booktitle = {Folia Medica Cassoviensia},
   keywords = {dorsal root ganglia; TNF-alpha; TNFR1},
   language = {eng},
   location = {Košice},
   isbn = {80-7097-607-1},
   pages = {22-22},
   publisher = {Institute of Neurobiology, Slovak Academy of Science},
   title = {Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain},
   year = {2005}
}

TY  - JOUR
ID  - 602084
AU  - Dubový, Petr - Jančálek, Radim - Klusáková, Ilona - Svíženská, Ivana - Pejchalová, Kateřina
PY  - 2005
TI  - Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain
PB  - Institute of Neurobiology, Slovak Academy of Science
CY  - Košice
SN  - 8070976071
KW  - dorsal root ganglia
KW  - TNF-alpha
KW  - TNFR1
N2  - Peripheral neuropathic pain has been investigated using rat peripheral nerve injury models including unilateral sciatic nerve transection or ligation, spinal nerve ligation or ventral root transection. The experimental models were utilized for the study of immunofluorescence (IF) staining for IL-1b, IL-6, TNFa and TNFaR1 in the rat DRG. Quantitative and qualitative IF changes in the DRG affected by unilateral nerve injury were compared with the contralateral ones removed from operated animals as well as naive rats. Ipsi- and contralateral DRG displayed increased IF for TNFa and TNFaR1 when compared with DRG of naive rats. The TNFa IF was increased in both DRG neurons and their satellite glial cells, while enhanced IF for TNFaR1 was observed only at the surface of satellite glial cells after various types of nerve injury. In addition, ipsilateral DRG exhibited an elevation of TNFa immunoreactivity by invaded ED-1+ macrophages. The IL-1b IF was increased in the DRG neurons following sciatic nerve constriction, but not after the nerve trasection. A higher level of IF for IL-6 was observed in the DRG neurons and their satellite glial cells following sciatic nerve ligature as well as ventral root transection.
ER  -

DUBOVÝ, Petr, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ivana SVÍŽENSKÁ and Kateřina PEJCHALOVÁ. Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain (Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain.). In \textit{Folia Medica Cassoviensia}. Košice: Institute of Neurobiology, Slovak Academy of Science, 2005, p.~22-22. ISBN~80-7097-607-1.

Detailed Information on Publication Record