| DVOŘÁK, Petr, E. BRYNDA, Jiří PACHERNÍK, M. HOUSKA and Z. PIENTKA. Surface immobilized protein multilayers for cell seeding. Langmuir. 2005, vol. 21, No 17, p. 7877-7883, 8 pp. ISSN 0743-7463. |
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@article{611465,
author = {Dvořák, Petr and Brynda, E. and Pacherník, Jiří and Houska, M. and Pientka, Z.},
article_number = {17},
keywords = {MOUSE EMBRYOS; BIOSENSORS; ALBUMIN; HEPARIN; INVITRO; PLASMA},
language = {eng},
issn = {0743-7463},
journal = {Langmuir},
title = {Surface immobilized protein multilayers for cell seeding},
volume = {21},
year = {2005}
}
TY - JOUR
ID - 611465
AU - Dvořák, Petr - Brynda, E. - Pacherník, Jiří - Houska, M. - Pientka, Z.
PY - 2005
TI - Surface immobilized protein multilayers for cell seeding
JF - Langmuir
VL - 21
IS - 17
SP - 7877-7883
EP - 7877-7883
SN - 07437463
KW - MOUSE EMBRYOS
KW - BIOSENSORS
KW - ALBUMIN
KW - HEPARIN
KW - INVITRO
KW - PLASMA
N2 - The adhesion and growth of mouse embryonic stem cells line D3 were tested in polystyrene culture dishes coated with the multilayer assemblies containing various cell-adhesive proteins such as gelatin, collagen IV, and laminin or polycations polylysine and poly(ethyleneimine). The cells were seeded in complete serum-containing media or in serum-free media and in the presence (non-differentiated) and/or absence (differentiated) of leukemia inhibitory factor. The cells grew best on surfaces coated with gelatin and collagen IV assemblies. There were no significant differences in the growth of the non-differentiated and differentiated cells in complete serum-containing media. When seeded in serum-free media, non-differentiated cells grew better than the differentiated ones. The layer-by-layer deposition appears to be a practicable technique by which scaffolds for tissue engineering can be coated with biomolecular assemblies tailored to specific cells and applications.
ER -
DVOŘÁK, Petr, E. BRYNDA, Jiří PACHERNÍK, M. HOUSKA and Z. PIENTKA. Surface immobilized protein multilayers for cell seeding. \textit{Langmuir}. 2005, vol.~21, No~17, p.~7877-7883, 8 pp. ISSN~0743-7463.
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